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pT7-Cas9-Nickase (D10A)

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SKU
GE100020
pT7-Cas9-Nickase vector (Cas9 D10A) (10 µg)
  $670.00
In Stock*

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TP790151 Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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Specifications
Specifications
Product Data
Function Cas9 Nickase
Features

T7 driven Cas9-Nickase expression vector

  • Serves as a template for T7 in vitro transcription of Cas9 D10A mRNA
  • Cas9 D10A expressed contains a C-terminal Myc-DDK tag
  • T7 driven gRNA can be produced from a separate vector, GE100025
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing
Noman, Aqeel, He
Front Plant Sci (2016) 7, 1740 DOI: 10.3389/fpls.2016.01740
Cited by 93
AI Summary: In the paper "CRISPR-Cas9: Tool for Qualitative and Quantitative Plant Genome Editing," OriGene products such as the Cas9 expression plasmid and guide RNA cloning kits were utilized to facilitate efficient CRISPR-Cas9-mediated genome editing in plants, enabling targeted gene modifications discussed in the review.
FigureFigureFigure
Showing 1-1 of 1 papers.
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