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DHRS9 (NM_005771) Human Over-expression Lysate

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SKU
LY429267
Transient overexpression lysate of dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208901
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005771, NP_005762
Synonyms 3ALPHA-HSD; RDH-TBE; RDH15; RDHL; RDHTBE; RETSDR8; SDR9C4
Predicted MW 35 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005762
Locus ID 10170
Cytogenetics 2q31.1
Protein Families WB
Protein Pathways Metabolic pathways, Retinol metabolism
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LC404693 Lyophilized DHRS9 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC417061 Lyophilized DHRS9 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC427994 Lyophilized DHRS9 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC427995 Lyophilized DHRS9 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC429267 Lyophilized DHRS9 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404693 Transient overexpression lysate of dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 2 (as WB positive control) 100 ug
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LY417061 Transient overexpression lysate of dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 1 (as WB positive control) 100 ug
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LY427994 Transient overexpression lysate of dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 3 (as WB positive control) 100 ug
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LY427995 Transient overexpression lysate of dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP320853 Recombinant protein of human dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 2, 20 µg 20 ug
$867.00
TP326895 Purified recombinant protein of Homo sapiens dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 3, 20 µg 20 ug
$867.00
TP326904 Purified recombinant protein of Homo sapiens dehydrogenase/reductase (SDR family) member 9 (DHRS9), transcript variant 4, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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