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MITF (NM_000248) Human Over-expression Lysate

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SKU
LY424990
Transient overexpression lysate of microphthalmia-associated transcription factor (MITF), transcript variant 4 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209561
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000248, NP_000239
Synonyms bHLHe32; CMM8; COMMAD; MI; WS2; WS2A
Predicted MW 46.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000239
Locus ID 4286
Cytogenetics 3p13
Protein Families WB
Protein Pathways Melanogenesis, Melanoma, Pathways in cancer
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Other "MITF" proteins (5)
SKU Description Size Price
LC404984 Lyophilized MITF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424990 Lyophilized MITF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404984 Transient overexpression lysate of microphthalmia-associated transcription factor (MITF), transcript variant 6 (as WB positive control) 100 ug
$665.00
TP761396 Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 5, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00
TP761494 Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 4, full length, with N-terminal HIS tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Detecting transcription factors with allosteric DNA-Silver nanocluster switches
Li, Chen, Wang et al
Anal. Chim. Acta (2019) 1048, 168-177 DOI: 10.1016/j.aca.2018.10.023
AI Summary: OriGene products, specifically Microphthalmia-associated transcription factor (MITF) and p65 proteins, were used as target transcription factors to validate the detection capabilities of the allosteric DNA-silver nanocluster switches described in the study.
Snippet: Microphthalmia-associated transcription factor (MITF, ∼46.8 kDa) and p65 (∼60 kDa) were purchased from OriGene Technologies (MD, U.S.A). Human serum albumin (HSA) and human α-thrombin were purchased from Sigma (MO, U.S.A.)
Showing 1-1 of 1 papers.
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