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MITF (NM_198158) Human Recombinant Protein

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SKU
TP761396
Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 5, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug
Size:
50 ug
  $270.00
In Stock*
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Frequently Bought Together (1)

TA314196 Rabbit polyclonal MITF (Ab-180/73) antibody
100 ul
$380.00

Other products for "MITF or NM_198158"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
A DNA sequence encoding human full-length MITF
Tag N-GST and C-His
Predicted MW 74.1 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_937801
Locus ID 4286
UniProt ID O75030
Cytogenetics 3p13
RefSeq Size 4454
RefSeq ORF 1239
Synonyms bHLHe32; CMM8; COMMAD; MI; WS2; WS2A
Summary The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. provided by RefSeq, Aug 2017
Protein Categories Cancer: Skin, Intracellular Proteins, Transciption Factors
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Melanogenesis, Melanoma, Pathways in cancer
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Other "MITF" proteins (5)
SKU Description Size Price
LC404984 Lyophilized MITF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424990 Lyophilized MITF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404984 Transient overexpression lysate of microphthalmia-associated transcription factor (MITF), transcript variant 6 (as WB positive control) 100 ug
$665.00
LY424990 Transient overexpression lysate of microphthalmia-associated transcription factor (MITF), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP761494 Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 4, full length, with N-terminal HIS tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Genetic insights into Tietz albinism-deafness syndrome: A new dominant-negative mutation in MITF
Yamamoto, Okamura, Wakamatsu et al
Pigment Cell Melanoma Res (2024)
Cited by 4
AI Summary: OriGene’s MITF (NM_198158) human recombinant protein (rhMITF; TP761396) was used as a positive control in electrophoretic mobility shift assays to assess the DNA-binding capability of MITF variants in the study.
Snippet: and/or an anti-MITF antibody (D5;Thermo Fisher). MITF (NM_198158) human recombinant protein (rhMITF;TP761396, Origene, Rockville, MD, USA) and protein extracted from BL21 cells transformed with an empty vector were used as positive and negative controls, respectively
MITF activity is regulated by a direct interaction with RAF proteins in melanoma cells
Estrada, Mirabal-Ortega, Méry et al
Commun Biol (2022) 5 (1), 101 DOI: 10.1038/s42003-022-03049-w
Cited by 15
AI Summary: OriGene’s human recombinant MITF protein and human recombinant flag-ARAF were used in co-immunoprecipitation experiments to demonstrate the direct interaction between MITF and RAF proteins in melanoma cells.
Snippet: … For immunoprecipitation of recombinant proteins, 15 ng of human recombinant MITF protein (Origene) were incubated overnight at 4 °C in NP40 Buffer with 75 ng of human recombinant flag-ARAF (Origene) and anti-flag magnetic beads (M2, Sigma). Coimmunoprecipitations are representative of;- , 15 ng of human recombinant MITF protein (Origene) were incubated overnight at 4 C in NP40 Buffer with 75 ng of human recombinant flag-ARAF (Origene) and anti-flag
FigureFigureFigure
New level of regulation of MITF activity by direct interaction with RAF proteins
Estrada, Mirabal-Ortega, Dingli et al
Research Square (2021)
Snippet: For immunoprecipitation of recombinant proteins, 15 ng of human recombinant MITF protein (Origene) were incubated overnight at 4 °C in NP40 Buffer with 75 ng of human recombinant flag-ARAF (Origene) and anti-flag magnetic beads (M2, Sigma)
A signal transduction approach for multiplexed detection of transcription factors by integrating DNA nanotechnology, multi-channeled isothermal amplification, and chromatography
Chen, Yan, Yang et al
J Chromatogr A (2020) 1624, 461148 DOI: 10.1016/j.chroma.2020.461148
Cited by 14
AI Summary: In this paper, OriGene products—including recombinant human p50 (TP303565), p53 (TP315322), AP-1 (TP305331), MITF (TP317566), and c-Myc (TP320236) proteins—were used as target transcription factors to validate and quantify the multiplexed detection capability of the described PAC system.
Detecting transcription factors with allosteric DNA-Silver nanocluster switches
Li, Chen, Wang et al
Analytica Chimica Acta (2018)
AI Summary: OriGene products, specifically the microphthalmia-associated transcription factor (MITF) and p65 proteins, were used as target transcription factors to validate the detection capability of the allosteric DNA-silver nanocluster switches described in the study.
Snippet: TATA box binding protein (TBP, ∼39 kDa) was obtained from ActiveMotif (CA, USA). Microphthalmia-associated transcription factor (MITF, ∼46.8 kDa) and p65 (∼60kDa) were purchased from OriGene Technologies (MD, USA)
Showing 1-5 of 5 papers.
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