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MITF (NM_000248) Human Over-expression Lysate

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SKU
LC424990
Lyophilized MITF HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209561
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000248, NP_000239
Synonyms bHLHe32; CMM8; COMMAD; MI; WS2; WS2A
Predicted MW 46.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_000239
Locus ID 4286
Cytogenetics 3p13
Protein Families WB
Protein Pathways Melanogenesis, Melanoma, Pathways in cancer
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Other "MITF" proteins (5)
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LC404984 Lyophilized MITF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY424990 Transient overexpression lysate of microphthalmia-associated transcription factor (MITF), transcript variant 4 (as WB positive control) 100 ug
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TP761396 Purified recombinant protein of Human microphthalmia-associated transcription factor (MITF), transcript variant 5, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
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file-alt Citations

Detecting transcription factors with allosteric DNA-Silver nanocluster switches
Li, Chen, Wang et al
Anal. Chim. Acta (2019) 1048, 168-177 DOI: 10.1016/j.aca.2018.10.023
AI Summary: OriGene products, specifically Microphthalmia-associated transcription factor (MITF) and p65 proteins, were used as target transcription factors to validate the detection capabilities of the allosteric DNA-silver nanocluster switches described in the study.
Snippet: Microphthalmia-associated transcription factor (MITF, ∼46.8 kDa) and p65 (∼60 kDa) were purchased from OriGene Technologies (MD, U.S.A). Human serum albumin (HSA) and human α-thrombin were purchased from Sigma (MO, U.S.A.)
Showing 1-1 of 1 papers.
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