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PAK4 (NM_001014831) Human Over-expression Lysate

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SKU
LY423086
Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206847
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001014831, NP_001014831
Predicted MW 64.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001014831
Locus ID 10298
Cytogenetics 19q13.2
Protein Families WB
Protein Pathways Axon guidance, ErbB signaling pathway, Focal adhesion, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
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Other "PAK4" proteins (15)
SKU Description Size Price
PH302302 PAK4 MS Standard C13 and N15-labeled recombinant protein (NP_005875) 10 ug
$3,360.00
PH306847 PAK4 MS Standard C13 and N15-labeled recombinant protein (NP_001014831) 10 ug
$3,360.00
PH317097 PAK4 MS Standard C13 and N15-labeled recombinant protein (NP_001014832) 10 ug
$3,360.00
LC417002 Lyophilized PAK4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423086 Lyophilized PAK4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423087 Lyophilized PAK4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC423088 Lyophilized PAK4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417002 Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY423087 Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY423088 Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 5 (as WB positive control) 100 ug
$665.00
TP302302 Recombinant protein of human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1, 20 µg 20 ug
$867.00
TP306847 Recombinant protein of human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 2, 20 µg 20 ug
$867.00
TP317097 Recombinant protein of human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 3, 20 µg 20 ug
$867.00
TP723905 Purified recombinant kinase domain protein of human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1, 10 µg 10 ug
$820.00
TP723906 Purified recombinant kinase domain protein of human p21 protein (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1, 100 µg 100 ug
$2,845.00

file-alt Citations

A novel interaction of PAK4 with PPARγ to regulate Nox1 and radiation-induced epithelial-to-mesenchymal transition in glioma
Kesanakurti, Maddirela, Banasavadi-Siddegowda et al
Oncogene (2017)
AI Summary: In this study, OriGene's full-length GFP-tagged PAK4 overexpression plasmid (PAK4-FL) was used to investigate the role of PAK4 in promoting mesenchymal transition and radioresistance in glioma cells.
Snippet: plasmid (PAK4sh) comprising a pool of three different targeting shRNAs (sc-39060-SH) and specific scrambled shRNA vector (SV) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), full-length GFP-tagged PAK4 overexpression plasmid (PAK4-FL) (Origene, ... All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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