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EMA (MUC1) (NM_001018016) Human Over-expression Lysate

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SKU
LY422694
Transient overexpression lysate of mucin 1, cell surface associated (MUC1), transcript variant 2 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221390
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001018016, NP_001018016
Synonyms ADMCKD; ADMCKD1; ADTKD2; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM
Predicted MW 28.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001018016
Locus ID 4582
Cytogenetics 1q22
Protein Families WB
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Other "EMA" proteins (10)
SKU Description Size Price
PH321390 MUC1 MS Standard C13 and N15-labeled recombinant protein (NP_001018016) 10 ug
$3,360.00
LC400880 Lyophilized MUC1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420828 Lyophilized MUC1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422694 Lyophilized MUC1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422695 Lyophilized MUC1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400880 Transient overexpression lysate of mucin 1, cell surface associated (MUC1), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY420828 Transient overexpression lysate of mucin 1, cell surface associated (MUC1), transcript variant 6 (as WB positive control) 100 ug
$436.00
LY422695 Transient overexpression lysate of mucin 1, cell surface associated (MUC1), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP321390 Recombinant protein of human mucin 1, cell surface associated (MUC1), transcript variant 2, 20 µg 20 ug
$737.00
TP760771 Purified recombinant protein of Human mucin 1, cell surface associated (MUC1), transcript variant 3, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Anti-Peptide Monoclonal Antibodies Generated for Immuno-Multiple Reaction Monitoring-Mass Spectrometry Assays Have a High Probability of Supporting Western blot and ELISA
Schoenherr, Saul, Whiteaker et al
Mol. Cell Proteomics (2015) 14 (2), 382-98 DOI: 10.1074/mcp.O114.043133
AI Summary: OriGene contributed overexpressed lysates, which were used as protein sources in the study to evaluate the performance of anti-peptide monoclonal antibodies in Western blot and ELISA assays.
Snippet: nci.nih.gov/index.html). The recombinant proteins were obtained from the Argonne National Laboratory (ANL, Lemont, IL), and the overexpressed lysates were purchased from Origene (Rockville, MD). The six NCI60 breast ... Cite SaveSaving
Showing 1-1 of 1 papers.
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