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SIRP alpha (SIRPA) (NM_001040023) Human Over-expression Lysate

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SKU
LY421879
Transient overexpression lysate of signal-regulatory protein alpha (SIRPA), transcript variant 2 (as WB positive control)
  • LY421879 is the same product as LY425671.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $665.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216320
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001040023, NP_001035112
Synonyms BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP
Predicted MW 54.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001035112
Locus ID 140885
Cytogenetics 20p13
Protein Families WB
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Other "SIRP alpha" proteins (13)
SKU Description Size Price
PH307315 SIRPA MS Standard C13 and N15-labeled recombinant protein (NP_542970) 10 ug
$3,360.00
PH316320 SIRPA MS Standard C13 and N15-labeled recombinant protein (NP_001035112) 10 ug
$3,360.00
PH322380 SIRPA MS Standard C13 and N15-labeled recombinant protein (NP_001035111) 10 ug
$3,360.00
LC409074 Lyophilized SIRPA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421878 Lyophilized SIRPA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC421879 Lyophilized SIRPA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY409074 Transient overexpression lysate of signal-regulatory protein alpha (SIRPA), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY421878 Transient overexpression lysate of signal-regulatory protein alpha (SIRPA), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP307315 Recombinant protein of human signal-regulatory protein alpha (SIRPA), transcript variant 3, 20 µg 20 ug
$737.00
TP316320 Recombinant protein of human signal-regulatory protein alpha (SIRPA), transcript variant 2, 20 µg 20 ug
$737.00
TP322380 Recombinant protein of human signal-regulatory protein alpha (SIRPA), transcript variant 1, 20 µg 20 ug
$737.00
TP723932 Recombinant Human SIRPa with C-terminal human Fc and 6×His tag 100 ug
$795.00
TP762372 Purified recombinant protein of Human signal-regulatory protein alpha (SIRPA), transcript variant 1, Glu31-Ile373, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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