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SIRP alpha (SIRPA) (NM_080792) Human Over-expression Lysate

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SKU
LC409074
Lyophilized SIRPA HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207315
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080792, NP_542970
Synonyms BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP
Predicted MW 54.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_542970
Locus ID 140885
Cytogenetics 20p13
Protein Families WB
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Other "SIRP alpha" proteins (13)
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PH307315 SIRPA MS Standard C13 and N15-labeled recombinant protein (NP_542970) 10 ug
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PH322380 SIRPA MS Standard C13 and N15-labeled recombinant protein (NP_001035111) 10 ug
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LC421878 Lyophilized SIRPA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC421879 Lyophilized SIRPA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY409074 Transient overexpression lysate of signal-regulatory protein alpha (SIRPA), transcript variant 3 (as WB positive control) 100 ug
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LY421878 Transient overexpression lysate of signal-regulatory protein alpha (SIRPA), transcript variant 1 (as WB positive control) 100 ug
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LY421879 Transient overexpression lysate of signal-regulatory protein alpha (SIRPA), transcript variant 2 (as WB positive control) 100 ug
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TP307315 Recombinant protein of human signal-regulatory protein alpha (SIRPA), transcript variant 3, 20 µg 20 ug
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TP316320 Recombinant protein of human signal-regulatory protein alpha (SIRPA), transcript variant 2, 20 µg 20 ug
$737.00
TP322380 Recombinant protein of human signal-regulatory protein alpha (SIRPA), transcript variant 1, 20 µg 20 ug
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TP723932 Recombinant Human SIRPa with C-terminal human Fc and 6×His tag 100 ug
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TP762372 Purified recombinant protein of Human signal-regulatory protein alpha (SIRPA), transcript variant 1, Glu31-Ile373, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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