TACD2 (TACSTD2) (NM_002353) Human Over-expression Lysate

SKU
LY419381
Transient overexpression lysate of tumor-associated calcium signal transducer 2 (TACSTD2) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202519
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002353, NP_002344
Synonyms EGP-1; EGP1; GA733-1; GA7331; GP50; M1S1; TROP2
Predicted MW 35.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002344
Locus ID 4070
Cytogenetics 1p32.1
Protein Families WB
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "TACD2" proteins (14)
SKU Description Size Price
PH302519 TACSTD2 MS Standard C13 and N15-labeled recombinant protein (NP_002344) 10 ug
$3,360.00
LC419381 Lyophilized TACSTD2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302519 Recombinant protein of human tumor-associated calcium signal transducer 2 (TACSTD2), 20 µg 20 ug
$737.00
TP721339 Human Trop2 Protein (C-His) 25 ug
$300.00
TP721340 Human Trop2 Protein (C-His-Avi) 25 ug
$300.00
TP721341 Biotinylated Human Trop2 Protein (C-His-Avi) 25 ug
$430.00
TP721342 PE Conjugated Human Trop2 Protein (C-His) 25 ug
$430.00
TP721343 APC Conjugated Human Trop2 Protein (C-His) 25 ug
$430.00
TP721344 Human Trop2 Protein (C-Fc) 25 ug
$300.00
TP721345 Human Trop2 Protein (C-Fc-Avi) 25 ug
$300.00
TP721346 Biotinylated Human Trop2 Protein (C-Fc-Avi) 25 ug
$430.00
TP721347 PE Conjugated Human Trop2 Protein (C-Fc) 25 ug
$430.00
TP721348 APC Conjugated Human Trop2 Protein (C-Fc) 25 ug
$430.00
TP724028 Recombinant human Trop2 protein with C-terminal mouse Fc and 6×His tag 100 ug
$770.00

file-alt Citations

TROP2 overexpression promotes proliferation and invasion of lung adenocarcinoma cells
Li, Jiang, Zhang
Biochem. Biophys. Res. Commun. (2016) 470 (1), 197-204 DOI: 10.1016/j.bbrc.2016.01.032
Cited by 64
AI Summary: OriGene’s TROP2 antibody (used at 1:3000 dilution) was utilized for Western blot analysis to assess TROP2 protein expression levels in lung adenocarcinoma cell lines in this study.
Snippet: The membranes were blocked in 5% skim milk and incubated with primary antibodies overnight (TROP2 1:3000, Origene, Rockville, MD, USA;b-actin 1:6000, Sigma) followed by horseradish peroxidase-conjugated anti-mouse antibodies (1:4000, Sigma–Aldrich). ... Cite SaveSaving
IHC WB
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products