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TACD2 (TACSTD2) (NM_002353) Human Over-expression Lysate

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SKU
LC419381
Lyophilized TACSTD2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202519
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002353, NP_002344
Synonyms EGP-1; EGP1; GA733-1; GA7331; GP50; M1S1; TROP2
Predicted MW 35.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002344
Locus ID 4070
Cytogenetics 1p32.1
Protein Families WB
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Other "TACD2" proteins (14)
SKU Description Size Price
PH302519 TACSTD2 MS Standard C13 and N15-labeled recombinant protein (NP_002344) 10 ug
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TP721339 Human Trop2 Protein (C-His) 25 ug
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TP721344 Human Trop2 Protein (C-Fc) 25 ug
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TP721345 Human Trop2 Protein (C-Fc-Avi) 25 ug
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TP721346 Biotinylated Human Trop2 Protein (C-Fc-Avi) 25 ug
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TP721347 PE Conjugated Human Trop2 Protein (C-Fc) 25 ug
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TP721348 APC Conjugated Human Trop2 Protein (C-Fc) 25 ug
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TP724028 Recombinant human Trop2 protein with C-terminal mouse Fc and 6×His tag 100 ug
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file-alt Citations

TROP2 overexpression promotes proliferation and invasion of lung adenocarcinoma cells
Li, Jiang, Zhang
Biochem. Biophys. Res. Commun. (2016) 470 (1), 197-204 DOI: 10.1016/j.bbrc.2016.01.032
Cited by 64
AI Summary: OriGene’s TROP2 antibody (used at 1:3000 dilution) was utilized for Western blot analysis to assess TROP2 protein expression levels in lung adenocarcinoma cell lines in this study.
Snippet: The membranes were blocked in 5% skim milk and incubated with primary antibodies overnight (TROP2 1:3000, Origene, Rockville, MD, USA;b-actin 1:6000, Sigma) followed by horseradish peroxidase-conjugated anti-mouse antibodies (1:4000, Sigma–Aldrich). ... Cite SaveSaving
IHC WB
Showing 1-1 of 1 papers.
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