CUL4A (NM_003589) Human Over-expression Lysate
SKU
LY418559
Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 2 (as WB positive control)
- LY418559 is the same product as LY429154.
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC203463 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_003589, NP_003580 |
| Predicted MW | 87.7 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_003580 |
| Locus ID | 8451 |
| Cytogenetics | 13q34 |
| Protein Families | WB |
| Protein Pathways | Nucleotide excision repair, Ubiquitin mediated proteolysis |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CUL4A" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303463 | CUL4A MS Standard C13 and N15-labeled recombinant protein (NP_003580) | 10 ug |
$3,360.00
|
|
| LC400388 | Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC418559 | Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400388 | Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| TP303463 | Purified recombinant protein of Homo sapiens cullin 4A (CUL4A), transcript variant 2, 20 µg | 20 ug |
$737.00
|
|
Citations
Recurrent Amplification at 13q34 Targets at CUL4A, IRS2, and TFDP1 As an Independent Adverse Prognosticator in Intrahepatic Cholangiocarcinoma
Liu, You, Weng et al
PLoS ONE (2015) 10 (12), e0145388 DOI: 10.1371/journal.pone.0145388
Liu, You, Weng et al
PLoS ONE (2015) 10 (12), e0145388 DOI: 10.1371/journal.pone.0145388
Cited by 10
AI Summary: In this study, OriGene products—including IRS2 cDNA (RC212413), specific siRNAs targeting IRS2, CUL4A, and TFDP1, and the PCNA antibody—were used for gene overexpression, gene silencing, and proliferation assays to investigate the roles of these genes in intrahepatic cholangiocarcinoma progression.
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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