OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

CUL4A (NM_003589) Human Over-expression Lysate

Be the first to review this product
SKU
LC418559
Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA321260 Anti-CUL4A Rabbit Polyclonal Antibody
100 ul
$305.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "CUL4A or NM_003589"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203463
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003589, NP_003580
Predicted MW 87.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_003580
Locus ID 8451
Cytogenetics 13q34
Protein Families WB
Protein Pathways Nucleotide excision repair, Ubiquitin mediated proteolysis
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "CUL4A" proteins (5)
SKU Description Size Price
PH303463 CUL4A MS Standard C13 and N15-labeled recombinant protein (NP_003580) 10 ug
$3,360.00
LC400388 Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400388 Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY418559 Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP303463 Purified recombinant protein of Homo sapiens cullin 4A (CUL4A), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

Recurrent Amplification at 13q34 Targets at CUL4A, IRS2, and TFDP1 As an Independent Adverse Prognosticator in Intrahepatic Cholangiocarcinoma
Liu, You, Weng et al
PLoS ONE (2015) 10 (12), e0145388 DOI: 10.1371/journal.pone.0145388
Cited by 10
AI Summary: In this study, OriGene products—including IRS2 cDNA (RC212413), specific siRNAs targeting IRS2, CUL4A, and TFDP1, and the PCNA antibody—were used for gene overexpression, gene silencing, and proliferation assays to investigate the roles of these genes in intrahepatic cholangiocarcinoma progression.
Snippet: No. RC212413) and specific siRNAs targeting IRS2, CUL4A and TFDP1 were purchased from Origene (Origene, MD, USA). ... Cellular proliferation was studied by analyzing proliferating cell nuclear antigen (PCNA;1;2000, Origene). ... Cite SaveSaving
WB IHC
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products