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CUL4A (NM_001008895) Human Over-expression Lysate

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SKU
LC400388
Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214798
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001008895, NP_001008895
Predicted MW 87.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001008895
Locus ID 8451
Cytogenetics 13q34
Protein Families WB
Protein Pathways Nucleotide excision repair, Ubiquitin mediated proteolysis
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Other "CUL4A" proteins (5)
SKU Description Size Price
PH303463 CUL4A MS Standard C13 and N15-labeled recombinant protein (NP_003580) 10 ug
$3,360.00
LC418559 Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400388 Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY418559 Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP303463 Purified recombinant protein of Homo sapiens cullin 4A (CUL4A), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

Autophagy Plays a Role in the CUL4A-Related Poor Prognosis of Intrahepatic Cholangiocarcinoma
Weng, Liu, Eng et al
Pathol Oncol Res (2021) 27, 602714 DOI: 10.3389/pore.2021.602714
AI Summary: OriGene products, including the CUL4A expression plasmid (RC207392) and CUL4A shRNA (TL312627), were used to manipulate CUL4A levels in intrahepatic cholangiocarcinoma cell lines, enabling the study of CUL4A’s role in regulating autophagy and its association with poor prognosis.
FigureFigureFigure
Showing 1-1 of 1 papers.
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