CUL4A Rabbit Polyclonal Antibody

SKU TA321260

Anti-CUL4A Rabbit Polyclonal Antibody

Product Data

• Application: IHC, WB
• Recommended Dilution: WB: 500-2000; WB positive control: HepG2, Hela, 231 cell and Human fetal brain tissue lysates; IHC: 40-200; Positive control: Human esophagus cancer; Predicted cell location: Nucleus
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 721-735 amino acids of Human cullin 4A
• Buffer: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 88 kDa
• Gene Name: cullin 4A
• Database Link:
  NP_001008895
  Q13619
• Background: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1; DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication.
• Synonyms: cullin 4A; OTTHUMP00000018753; OTTHUMP00000018754

Reference Data

• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis