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PP2A-beta (PPP2CB) (NM_004156) Human Over-expression Lysate

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SKU
LY418183
Transient overexpression lysate of protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219083
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004156, NP_004147
Synonyms AI115466; D8Ertd766e; PP2Ac; PP2CB; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2a, catalytic subunit, beta isoform
Predicted MW 35.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004147
Locus ID 5516
Cytogenetics 8p12
Protein Families WB
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
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Other "PP2A-beta" proteins (4)
SKU Description Size Price
LC418183 Lyophilized PPP2CB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422909 Lyophilized PPP2CB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422909 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP760699 Purified recombinant protein of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Unlocking repression of the human luteinizing hormone receptor gene by trichostatin A-induced cell-specific phosphatase release
Zhang, Liao, Dufau
J Biol Chem (2008) 283 (35), 24039-46 DOI: 10.1074/jbc.M801878200
AI Summary: In this study, OriGene's PP2A (Cat# SC119785) and PP1 (Cat# SC119785) antibodies were used to investigate the roles of these phosphatases in TSA-induced activation of LHR gene transcription by enabling detection and analysis of their association with the LHR promoter.
Showing 1-1 of 1 papers.
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