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PP2A-beta (PPP2CB) (NM_004156) Human Over-expression Lysate

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SKU
LC418183
Lyophilized PPP2CB HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219083
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004156, NP_004147
Synonyms AI115466; D8Ertd766e; PP2Ac; PP2CB; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2a, catalytic subunit, beta isoform
Predicted MW 35.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_004147
Locus ID 5516
Cytogenetics 8p12
Protein Families WB
Protein Pathways Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
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Other "PP2A-beta" proteins (4)
SKU Description Size Price
LC422909 Lyophilized PPP2CB HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418183 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY422909 Transient overexpression lysate of protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP760699 Purified recombinant protein of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

file-alt Citations

Unlocking repression of the human luteinizing hormone receptor gene by trichostatin A-induced cell-specific phosphatase release
Zhang, Liao, Dufau
J Biol Chem (2008) 283 (35), 24039-46 DOI: 10.1074/jbc.M801878200
AI Summary: In this study, OriGene's PP2A (Cat# SC119785) and PP1 (Cat# SC119785) antibodies were used to investigate the roles of these phosphatases in TSA-induced activation of LHR gene transcription by enabling detection and analysis of their association with the LHR promoter.
Showing 1-1 of 1 papers.
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