PP2A-beta (PPP2CB) (NM_004156) Human Tagged ORF Clone
SKU
RC219083
PPP2CB (Myc-DDK-tagged)-Human protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PP2A-beta |
| Synonyms | AI115466; D8Ertd766e; PP2Ac; PP2CB; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2a, catalytic subunit, beta isoform |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004156 |
| ORF Size | 927 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004156.2, NP_004147.1 |
| RefSeq Size | 1807 bp |
| RefSeq ORF | 929 bp |
| Locus ID | 5516 |
| Cytogenetics | 8p12 |
| Domains | Metallophos, PP2Ac |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway |
| MW | 35.4 kDa |
| Summary | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. provided by RefSeq, Mar 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004156" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC219083L3 | Lenti ORF clone of Human protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC219083L4 | Lenti ORF clone of Human protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG219083 | PPP2CB (tGFP-tagged) - Human protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC117555 | PPP2CB (untagged)-Human protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
Defects of protein phosphatase 2A causes corticosteroid insensitivity in severe asthma
Kobayashi, Mercado, Barnes et al
PLoS ONE (2011) 6 (12), e27627 DOI: 10.1371/journal.pone.0027627
Kobayashi, Mercado, Barnes et al
PLoS ONE (2011) 6 (12), e27627 DOI: 10.1371/journal.pone.0027627
Cited by 100
AI Summary: OriGene’s pCMV6 Entry plasmid containing the human PP2A catalytic subunit, alpha isoform, was used to overexpress PP2A in U937 cells, enabling the study of PP2A’s role in regulating corticosteroid sensitivity in severe asthma.
Snippet: Furthermore, we reported first time that PP2A was down-regulated in PBMCs from severe asthmatics with a concomitant increase in ... 2 µg of DNA/plasmids (pCMV6 Entry, OriGene Technologies, Rockville, MD) containing the human PP2A catalytic subunit, alpha isoform (PP2A
Showing 1-1 of 1 papers.
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