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HMGCS2 (NM_005518) Human Over-expression Lysate

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SKU
LY417253
Transient overexpression lysate of 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208128
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005518, NP_005509
Predicted MW 56.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005509
Locus ID 3158
Cytogenetics 1p12
Protein Families WB
Protein Pathways Butanoate metabolism, leucine and isoleucine degradation, Metabolic pathways, PPAR signaling pathway, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Valine
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Other "HMGCS2" proteins (6)
SKU Description Size Price
PH308128 HMGCS2 MS Standard C13 and N15-labeled recombinant protein (NP_005509) 10 ug
$3,360.00
LC417253 Lyophilized HMGCS2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431416 Lyophilized HMGCS2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY431416 Transient overexpression lysate of 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 2 (as WB positive control) 100 ug
$436.00
TP308128 Recombinant protein of human 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, 20 µg 20 ug
$867.00
TP761046 Purified recombinant protein of Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 2, full length, with N-terminal HIS tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Cancer-associated fibroblasts promote prostate tumor growth and progression through upregulation of cholesterol and steroid biosynthesis
Neuwirt, Bouchal, Kharaishvili et al
Cell Commun. Signal (2020) 18 (1), 11 DOI: 10.1186/s12964-019-0505-5
Cited by 110
AI Summary: OriGene’s pcDNA3-HMGCS2 plasmid was used to overexpress HMGCS2 in LNCaP prostate cancer cells, enabling the study to assess the functional role of this gene in cholesterol and steroid biosynthesis pathways promoted by cancer-associated fibroblasts.
Snippet: Exogenous overexpression of HMGCS2. HMGCS2 was overexpressed in LNCaP cells, which exhibit low to undetectable expression of HMGCS2 using a pcDNA3-HMGCS2 plasmid (Origene). Cells (500,000 cells/well) were seeded into a 6-well plate and incubated;Exogenous overexpression of HMGCS2. HMGCS2 was overexpressed in LNCaP cells, which exhibit low to undetectable expression of HMGCS2 using a pcDNA3-HMGCS2 plasmid (Origene). Cells (500,000 cells/well) were seeded into a 6-well plate and incubated overnight;;- Exogenous overexpression of HMGCS2. HMGCS2 was overexpressed in LNCaP cells, which exhibit low to undetectable expression of HMGCS2 using a pcDNA3-HMGCS2 plasmid (Origene). …
FigureFigureFigure
Showing 1-1 of 1 papers.
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