HMGCS2 (NM_005518) Human Tagged ORF Clone
SKU
RC208128
HMGCS2 (Myc-DDK-tagged)-Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1
$473.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HMGCS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005518 |
| ORF Size | 1524 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005518.4 |
| RefSeq Size | 2477 bp |
| RefSeq ORF | 1527 bp |
| Locus ID | 3158 |
| UniProt ID | P54868 |
| Cytogenetics | 1p12 |
| Domains | HMG_CoA_synt |
| Protein Families | Druggable Genome |
| Protein Pathways | Butanoate metabolism, leucine and isoleucine degradation, Metabolic pathways, PPAR signaling pathway, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Valine |
| MW | 56.6 kDa |
| Summary | The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Oct 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005518" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208128L3 | Lenti ORF clone of Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged | 10 ug |
$773.00
|
|
| RC208128L4 | Lenti ORF clone of Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged | 10 ug |
$773.00
|
|
| RG208128 | HMGCS2 (tGFP-tagged) - Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$489.00
$673.00
|
|
| SC116704 | HMGCS2 (untagged)-Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1 | 10 ug |
$474.00
|
|
Citations
Ascites protects against ferroptosis and enables the peritoneal growth of ovarian cancer
Setayeshpour, Chen, Dayanidhi et al
Nat Commun (2026) 17 (1) DOI: 10.1038/s41467-026-72116-1
Setayeshpour, Chen, Dayanidhi et al
Nat Commun (2026) 17 (1) DOI: 10.1038/s41467-026-72116-1
Cited by 4
AI Summary: OriGene products contributed to this study by providing the mouse HMGCS2 ORF clone in the pCMV6-Entry backbone (MG208162 and RC208128) for overexpression experiments and the lentiviral vector (PS100069) for generating stable cell lines, enabling functional analyses of HMGCS2 in ovarian cancer ferroptosis resistance.
Snippet: … For transient overexpression of HMGCS2, 100 ng of mouse _hmgcs2_ ORF clone with a pCMV6-Entry backbone (Origene, MG208162) was diluted in Opti-MEM I (9 µL). 0.3 µL of the TransIT -LT1 transfection reagent (Mirus, MIR2305) was added before the mix was incubated for 15 min in a 96-well plate;plasmids was conducted using the Qiagen Plasmid Midi kit (12145). For transient overexpression of HMGCS2, 100 ng of mouse hmgcs2 ORF clone with a pCMV6-Entry backbone (Origene, MG208162) was diluted in Opti-MEM I (9 µL). 0.3 µL of the;- HMGCS2 ORF clone with a pCMV6-Entry backbone (Origene, RC208128) was cut and ligated into a lentiviral vector (Origene, PS100069) according to the manufacturer’s
Ketogenesis attenuates KLF5-dependent production of CXCL12 to overcome the immunosuppressive tumor microenvironment in colorectal cancer
Wei, Zhou, Li et al
Cancer Res (2022) 82 (8), 1575-1588 DOI: 10.1158/0008-5472.CAN-21-2778
Wei, Zhou, Li et al
Cancer Res (2022) 82 (8), 1575-1588 DOI: 10.1158/0008-5472.CAN-21-2778
Ketogenesis alleviates TNFα-induced apoptosis and inflammatory responses in intestinal cells
Kim, Napier, Kim et al
Free Radic Biol Med (2021) 172, 90-100 DOI: 10.1016/j.freeradbiomed.2021.05.032
Kim, Napier, Kim et al
Free Radic Biol Med (2021) 172, 90-100 DOI: 10.1016/j.freeradbiomed.2021.05.032
Cited by 52
AI Summary: OriGene products contributed to this study by providing HMGCS2 shRNA (for gene knockdown) and HMGCS2 overexpression plasmids, which were used to manipulate HMGCS2 levels in intestinal epithelial cells to investigate the role of ketogenesis in TNFα-induced apoptosis and inflammation.
Ketogenesis alleviates TNFα-induced apoptosis and inflammatory responses in intestinal cells
Kim, Napier, Kim et al
Free Radical Biology and Medicine (2021) 172, 90-100 DOI: 10.1016/j.freeradbiomed.2021.05.032
Kim, Napier, Kim et al
Free Radical Biology and Medicine (2021) 172, 90-100 DOI: 10.1016/j.freeradbiomed.2021.05.032
Cited by 52
AI Summary: OriGene products, specifically the pCMV6-Entry-HMGCS2 cDNA clone (RC208128) and the pCMV6-Entry empty vector (PS100001), were used to generate stable cell lines overexpressing or serving as controls for HMGCS2, enabling the study of ketogenesis in TNFα-induced apoptosis and inflammation in intestinal cells.
Snippet: To establish stable cell lines expressing HMGCS2, pCMV6-Entry-HMGCS2 cDNA clone (RC208128, OriGene Technologies, Rockville, MD) or pCMV6-Entry [empty vector (EV), PS100001, OriGene] were transfected into DLD1 or HT29 cells using Lipofectamine 2000;To establish stable cell lines expressing HMGCS2, pCMV6-Entry-HMGCS2 cDNA clone (RC208128, OriGene Technologies,
Idi1 and Hmgcs2 Are Affected by Stretch in HL-1 Atrial Myocytes
Fang, Chen, Chang et al
Int J Mol Sci (2018) 19 (12) DOI: 10.3390/ijms19124094
Fang, Chen, Chang et al
Int J Mol Sci (2018) 19 (12) DOI: 10.3390/ijms19124094
AI Summary: OriGene’s pCMV6-Hmgcs2 (Myc-DDK-tagged) plasmid was used to transfect HL-1 atrial myocytes in this study, enabling the investigation of Hmgcs2’s regulatory role in stretch-induced changes in lipid metabolism.
Snippet: The pCMV6-Hmgcs2 (Myc-DDK-tagged) was cloned by OriGene (Rockville, MD, USA) with Sgf I-Rsr II as restriction sites. The HL-1 atrial myocytes were transfected with lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA). The lipofectamine 2000 was added to cells grown in serum-free Claycomb medium and cells were incubated for 8 h at 37 °C
HMGCS2 promotes autophagic degradation of the amyloid-β precursor protein through ketone body-mediated mechanisms
Hu, Zhu, Lai et al
Biochem. Biophys. Res. Commun. (2017)
Hu, Zhu, Lai et al
Biochem. Biophys. Res. Commun. (2017)
AI Summary: In this study, the HMGCS2 plasmid from OriGene was used to overexpress HMGCS2 in cells, enabling the authors to investigate its role in promoting autophagic degradation of amyloid-β precursor protein through ketone body-mediated mechanisms.
Snippet: toxicity [16]. 2.3. Plasmid and small interfering RNA (siRNA) transfection. The HMGCS2 plasmid was purchased from Origene (Rockville, MD), and an empty vector was used as the control (referred to here as Vector). The cells ... All 2 versions Cite SaveSaving
Showing 1-6 of 7 papers.
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