HMGCS2 (NM_005518) Human Over-expression Lysate
SKU
LC417253
Lyophilized HMGCS2 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC208128 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_005518, NP_005509 |
| Predicted MW | 56.6 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_005509 |
| Locus ID | 3158 |
| Cytogenetics | 1p12 |
| Protein Families | WB |
| Protein Pathways | Butanoate metabolism, leucine and isoleucine degradation, Metabolic pathways, PPAR signaling pathway, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Valine |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "HMGCS2" proteins (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH308128 | HMGCS2 MS Standard C13 and N15-labeled recombinant protein (NP_005509) | 10 ug |
$3,360.00
|
|
| LC431416 | Lyophilized HMGCS2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY417253 | Transient overexpression lysate of 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY431416 | Transient overexpression lysate of 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| TP308128 | Recombinant protein of human 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, 20 µg | 20 ug |
$867.00
|
|
| TP761046 | Purified recombinant protein of Human 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (HMGCS2), nuclear gene encoding mitochondrial protein, transcript variant 2, full length, with N-terminal HIS tag, expressed in E.coli, 50ug | 50 ug |
$270.00
|
|
Citations
Cancer-associated fibroblasts promote prostate tumor growth and progression through upregulation of cholesterol and steroid biosynthesis
Neuwirt, Bouchal, Kharaishvili et al
Cell Commun. Signal (2020) 18 (1), 11 DOI: 10.1186/s12964-019-0505-5
Neuwirt, Bouchal, Kharaishvili et al
Cell Commun. Signal (2020) 18 (1), 11 DOI: 10.1186/s12964-019-0505-5
Cited by 110
AI Summary: OriGene’s pcDNA3-HMGCS2 plasmid was used to overexpress HMGCS2 in LNCaP prostate cancer cells, enabling the study to assess the functional role of this gene in cholesterol and steroid biosynthesis pathways promoted by cancer-associated fibroblasts.
Snippet: Exogenous overexpression of HMGCS2. HMGCS2 was overexpressed in LNCaP cells, which exhibit low to undetectable expression of HMGCS2 using a pcDNA3-HMGCS2 plasmid (Origene). Cells (500,000 cells/well) were seeded into a 6-well plate and incubated;Exogenous overexpression of HMGCS2. HMGCS2 was overexpressed in LNCaP cells, which exhibit low to undetectable expression of HMGCS2 using a pcDNA3-HMGCS2 plasmid (Origene). Cells (500,000 cells/well) were seeded into a 6-well plate and incubated overnight;;- Exogenous overexpression of HMGCS2. HMGCS2 was overexpressed in LNCaP cells, which exhibit low to undetectable expression of HMGCS2 using a pcDNA3-HMGCS2 plasmid (Origene). …
Showing 1-1 of 1 papers.
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