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PD-L1 (CD274) (NM_014143) Human Over-expression Lysate

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SKU
LY415473
Transient overexpression lysate of PD-L1 / CD274 molecule (PD-L1 / CD274) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213071
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014143, NP_054862
Synonyms B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
Predicted MW 33.28 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_054862
Locus ID 29126
Cytogenetics 9p24.1
Protein Families WB
Protein Pathways Cell adhesion molecules (CAMs)
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file-alt Citations

The prognostic role of immune checkpoint markers programmed cell death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) in a large, multicenter prostate cancer cohort
Ness, Andersen, Khanehkenari et al
Oncotarget (2017) 8 (16), 26789-26801 DOI: 10.18632/oncotarget.15817
Cited by 82
AI Summary: OriGene products, specifically the anti-PD-1 (Cat# TA807127) and anti-PD-L1 (Cat# TA592207) antibodies, were used for immunohistochemical detection of PD-1 and PD-L1 expression in prostate cancer tissue samples, enabling the study’s analysis of their prognostic significance.
FigureFigureFigure
Assessing PDL-1 and PD-1 in Non-Small Cell Lung Cancer: A Novel Immunoscore Approach
Paulsen, Kilvaer, Khanehkenari et al
Clin Lung Cancer (2017) 18 (2), 220-233.e8 DOI: 10.1016/j.cllc.2016.09.009
Cited by 88
AI Summary: In this study, OriGene’s anti-PD-L1 antibody (TA307034) and anti-PD-1 antibody (TA811276) were used for immunohistochemical evaluation of PD-L1 and PD-1 expression in tumor samples, enabling the assessment of their prognostic value in non-small cell lung cancer.
The tumor suppressor miR-138-5p targets PD-L1 in colorectal cancer
Zhao, Yu, Yi et al
Oncotarget (2016) 7 (29), 45370-45384 DOI: 10.18632/oncotarget.9659
FigureFigureFigure
The prognostic role of immune checkpoint markers programmed cell death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) in a large, multicenter prostate cancer cohort
Ness, Andersen, Khanehkenari et al
oncotarget (2016)
Snippet: Overexpressed human HEK293T cell lysates were utilized from OriGene for PD-L1 (#LY415473), PD-1 (#LY401555) and HEK293 as empty vector (#LY500001/ negative control). Cells were incubated with 2xSDS sample buffer ... Cite SaveSaving
Assessing PD-L1 and PD-1 in Non-Small Cell Lung Cancer (NSCLC): a Novel Immunoscore Approach
Paulsen, Kilvaer, Khanehkenari et al
Clinical Lung Cancer (2016)
AI Summary: OriGene products, including PD-L1 lysate (#LY415473), PD-1 lysate (#LY401555), HEK293 lysate (#LY500001/negative control), and 2xSDS Sample Buffer, were used as controls and for sample preparation in the assessment of PD-L1 and PD-1 expression in NSCLC.
Snippet: Transient overexpressed human HEK293T cell lysates were utilized from OriGenefor PD-L1(#LY415473), PD-1(#LY401555) and HEK293(#LY500001/negative control);they wereincubated with 2xSDS Sample Buffer (OriGene) for 10 minutes at 100C. Equal amounts ... Cite SaveSaving
Showing 1-5 of 5 papers.
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