OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

PD-L1 (CD274) (NM_014143) Human Over-expression Lysate

Be the first to review this product
SKU
LY415473
Transient overexpression lysate of PD-L1 / CD274 molecule (PD-L1 / CD274) (as WB positive control)
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA507087 PD-L1 / CD274 mouse monoclonal antibody, clone OTI2C7 (formerly 2C7)
100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "PD-L1 or NM_014143"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213071
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014143, NP_054862
Synonyms B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
Predicted MW 33.28 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_054862
Locus ID 29126
Cytogenetics 9p24.1
Protein Families WB
Protein Pathways Cell adhesion molecules (CAMs)
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "PD-L1" proteins (19)
SKU Description Size Price
LC415473 Lyophilized CD274 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP700200 Purified recombinant protein of Homo sapiens programmed cell death 1 ligand 1(PD-L1/CD274), transcript variant 1, residue 19-239aa, with C-terminal DDK/His tag, expressed in HEK293 cells. 20 ug
$900.00
TP700201 Purified recombinant protein of Homo sapiens programmed cell death 1 ligand 1 (PD-L1/CD274), residues 19-239aa, with C-terminal Fc tag, expressed in HEK293 cells. 20 ug
$900.00
TP710226 Purified recombinant protein of Human CD274 molecule (CD274), residues 19-290aa, with C-terminal DDK tag, expressed in sf9 insect cells 20 ug
$540.00
TP710227 Purified recombinant protein of Homo sapiens programmed cell death 1 ligand 1(PD-L1/CD274), transcript variant 1, extracellular domain residue (19-238aa) with C-terminal DDK tag, expressed and secreted from insect sf9 cells, 20ug 20 ug
$540.00
TP720630 Purified recombinant protein of Human PD-L1 / CD274 molecule (PD-L1 / CD274) 10 ug
$175.00
TP721254 Human PD-L1 Protein (C-His) 25 ug
$300.00
TP721255 Human PD-L1 Protein (C-His-Avi) 25 ug
$300.00
TP721256 Biotinylated Human PD-L1 Protein (C-His-Avi) 25 ug
$430.00
TP721257 PE Conjugated Human PD-L1 Protein (C-His) 25 ug
$430.00
TP721258 APC Conjugated Human PD-L1 Protein (C-His) 25 ug
$430.00
TP721259 Human PD-L1 Protein (C-Fc) 25 ug
$300.00
TP721260 Human PD-L1 Protein (C-Fc-Avi) 25 ug
$300.00
TP721261 Biotinylated Human PD-L1 Protein (C-Fc-Avi) 25 ug
$430.00
TP721262 PE Conjugated Human PD-L1 Protein (C-Fc) 25 ug
$430.00
TP721263 APC Conjugated Human PD-L1 Protein (C-Fc) 25 ug
$430.00
TP723946 Recombinant human PD-L1 protein with C-terminal mouse Fc and 6×His tag 100 ug
$905.00
TP724015 Recombinant human PD-L1 protein with C-terminal 6×His tag 100 ug
$700.00
TP762432 Purified recombinant protein of Human PD-L1 / CD274 molecule (PD-L1 / CD274), Trp13-Thr239, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$240.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products