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ADAR1 (ADAR) (NM_015841) Human Over-expression Lysate

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SKU
LY414369
Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 3 (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC223644
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015841, NP_056656
Synonyms ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
Predicted MW 130.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_056656
Locus ID 103
Cytogenetics 1q21.3
Protein Families WB
Protein Pathways Cytosolic DNA-sensing pathway
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Other "ADAR1" proteins (10)
SKU Description Size Price
PH319761 ADAR MS Standard C13 and N15-labeled recombinant protein (NP_001020278) 10 ug
$3,360.00
LC414369 Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC420128 Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422591 Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC425483 Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY420128 Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY422591 Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 4 (as WB positive control) 100 ug
$665.00
LY425483 Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 4 (as WB positive control) 100 ug
$665.00
TP319761 Recombinant protein of human adenosine deaminase, RNA-specific (ADAR), transcript variant 4, 20 µg 20 ug
$867.00
TP762358 Purified recombinant protein of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 4, Thr30-Gln360, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

RNA modifications can affect RNase H1-mediated PS-ASO activity
Doxtader Lacy, Liang, Zhang et al
Mol Ther Nucleic Acids (2022) 28, 814-828 DOI: 10.1016/j.omtn.2022.05.024
Cited by 33
AI Summary: In this study, OriGene’s RNASEH1 cDNA clone (Cat# SC119513) was used to express recombinant RNase H1, enabling the authors to assess how RNA modifications influence RNase H1-mediated cleavage activity of PS-ASOs.
FigureFigureFigure
Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria
Liu, Wang, Wu et al
BMC Med. Genet. (2014) 15 (1), 69 DOI: 10.1186/1471-2350-15-69
Cited by 23
AI Summary: OriGene’s pCMV6-ADAR1 plasmid was used as the source of wild-type ADAR1 coding sequence for in vitro expression assays to study the effects of novel ADAR1 mutations in this paper.
Snippet: In vitro expression assay The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into pFLAG-CMV-2 (Sigma-Aldrich, St. Louis, MO, USA) at its Not I restriction site by standard procedure. ... Cite SaveSaving
Showing 1-2 of 2 papers.
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