OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

ADAR1 (ADAR) (NM_015841) Human Over-expression Lysate

Be the first to review this product
SKU
LC414369
Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA308833 Rabbit polyclonal antibody to Adenosine Deaminase (adenosine deaminase, RNA-specific)
100 ul
$665.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "ADAR1 or NM_015841"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC223644
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_015841, NP_056656
Synonyms ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
Predicted MW 130.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_056656
Locus ID 103
Cytogenetics 1q21.3
Protein Families WB
Protein Pathways Cytosolic DNA-sensing pathway
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "ADAR1" proteins (10)
SKU Description Size Price
PH319761 ADAR MS Standard C13 and N15-labeled recombinant protein (NP_001020278) 10 ug
$3,360.00
LC420128 Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422591 Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC425483 Lyophilized ADAR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY414369 Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY420128 Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY422591 Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 4 (as WB positive control) 100 ug
$665.00
LY425483 Transient overexpression lysate of adenosine deaminase, RNA-specific (ADAR), transcript variant 4 (as WB positive control) 100 ug
$665.00
TP319761 Recombinant protein of human adenosine deaminase, RNA-specific (ADAR), transcript variant 4, 20 µg 20 ug
$867.00
TP762358 Purified recombinant protein of Human adenosine deaminase, RNA-specific (ADAR), transcript variant 4, Thr30-Gln360, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

RNA modifications can affect RNase H1-mediated PS-ASO activity
Doxtader Lacy, Liang, Zhang et al
Mol Ther Nucleic Acids (2022) 28, 814-828 DOI: 10.1016/j.omtn.2022.05.024
Cited by 33
AI Summary: In this study, OriGene’s RNASEH1 cDNA clone (Cat# SC119513) was used to express recombinant RNase H1, enabling the authors to assess how RNA modifications influence RNase H1-mediated cleavage activity of PS-ASOs.
FigureFigureFigure
Five novel mutations in the ADAR1 gene associated with dyschromatosis symmetrica hereditaria
Liu, Wang, Wu et al
BMC Med. Genet. (2014) 15 (1), 69 DOI: 10.1186/1471-2350-15-69
Cited by 23
AI Summary: OriGene’s pCMV6-ADAR1 plasmid was used as the source of wild-type ADAR1 coding sequence for in vitro expression assays to study the effects of novel ADAR1 mutations in this paper.
Snippet: In vitro expression assay The wild-type coding sequence of ADAR1 was amplified from pCMV6-ADAR1 (Origene) by PCR and subcloned into pFLAG-CMV-2 (Sigma-Aldrich, St. Louis, MO, USA) at its Not I restriction site by standard procedure. ... Cite SaveSaving
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products