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NEIL1 (NM_024608) Human Over-expression Lysate

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SKU
LY411199
Transient overexpression lysate of nei endonuclease VIII-like 1 (E. coli) (NEIL1) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202329
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_024608, NP_078884
Synonyms FPG1; hFPG1; NEI1
Predicted MW 43.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_078884
Locus ID 79661
Cytogenetics 15q24.2
Protein Families WB
Protein Pathways Base excision repair
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Other "NEIL1" proteins (4)
SKU Description Size Price
PH302329 NEIL1 MS Standard C13 and N15-labeled recombinant protein (NP_078884) 10 ug
$3,360.00
LC411199 Lyophilized NEIL1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302329 Purified recombinant protein of Homo sapiens nei endonuclease VIII-like 1 (E. coli) (NEIL1), 20 µg 20 ug
$867.00
TP762007 Purified recombinant protein of Human nei endonuclease VIII-like 1 (E. coli) (NEIL1),Leu186-Ser390, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Concordance of hydrogen peroxide-induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors
Jin, Meng, Johnson et al
Sci Adv (2022) 8 (22), eabn3815 DOI: 10.1126/sciadv.abn3815
Cited by 44
AI Summary: OriGene’s NEIL1 DNA glycosylase was used in this study to evaluate oxidative DNA base damage, helping to map patterns of 8-oxo-guanine relevant to cancer mutation signatures.
Snippet: …DNA base damage was evaluated by treatment with Endo III (NEB) and/or NEIL1 (OriGene, Rockville, MD) DNA glycosylases, followed by Endo IV and S1 treatment, and then observed on a 1% agarose gel. … DNA base damage was evaluated by treatment with Endo III (NEB) and/or NEIL1 (OriGene, Rockville, MD) DNA glycosylases, followed by Endo IV and S1 treatment, and then observed on a 1% agarose gel
Showing 1-1 of 1 papers.
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