OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

NEIL1 (NM_024608) Human Over-expression Lysate

Be the first to review this product
SKU
LC411199
Lyophilized NEIL1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA800344 NEIL1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "NEIL1 or NM_024608"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202329
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_024608, NP_078884
Synonyms FPG1; hFPG1; NEI1
Predicted MW 43.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_078884
Locus ID 79661
Cytogenetics 15q24.2
Protein Families WB
Protein Pathways Base excision repair
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "NEIL1" proteins (4)
SKU Description Size Price
PH302329 NEIL1 MS Standard C13 and N15-labeled recombinant protein (NP_078884) 10 ug
$3,360.00
LY411199 Transient overexpression lysate of nei endonuclease VIII-like 1 (E. coli) (NEIL1) (as WB positive control) 100 ug
$436.00
TP302329 Purified recombinant protein of Homo sapiens nei endonuclease VIII-like 1 (E. coli) (NEIL1), 20 µg 20 ug
$867.00
TP762007 Purified recombinant protein of Human nei endonuclease VIII-like 1 (E. coli) (NEIL1),Leu186-Ser390, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Concordance of hydrogen peroxide-induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors
Jin, Meng, Johnson et al
Sci Adv (2022) 8 (22), eabn3815 DOI: 10.1126/sciadv.abn3815
Cited by 44
AI Summary: OriGene’s NEIL1 DNA glycosylase was used in this study to evaluate oxidative DNA base damage, helping to map patterns of 8-oxo-guanine relevant to cancer mutation signatures.
Snippet: …DNA base damage was evaluated by treatment with Endo III (NEB) and/or NEIL1 (OriGene, Rockville, MD) DNA glycosylases, followed by Endo IV and S1 treatment, and then observed on a 1% agarose gel. … DNA base damage was evaluated by treatment with Endo III (NEB) and/or NEIL1 (OriGene, Rockville, MD) DNA glycosylases, followed by Endo IV and S1 treatment, and then observed on a 1% agarose gel
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products