ZSCAN4 (NM_152677) Human Over-expression Lysate
SKU
LY407355
Transient overexpression lysate of zinc finger and SCAN domain containing 4 (ZSCAN4) (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC211003 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_152677, NP_689890 |
| Synonyms | ZNF494 |
| Predicted MW | 49 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_689890 |
| Locus ID | 201516 |
| Cytogenetics | 19q13.43 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "ZSCAN4" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH311003 | ZSCAN4 MS Standard C13 and N15-labeled recombinant protein (NP_689890) | 10 ug |
$3,360.00
|
|
| LC407355 | Lyophilized ZSCAN4 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP311003 | Recombinant protein of human zinc finger and SCAN domain containing 4 (ZSCAN4), 20 µg | 20 ug |
$737.00
|
|
Citations
ZSCAN4 facilitates chromatin remodeling and promotes the cancer stem cell phenotype
Portney, Arad, Gupta et al
Oncogene (2020) 39 (26), 4970-4982 DOI: 10.1038/s41388-020-1333-1
Portney, Arad, Gupta et al
Oncogene (2020) 39 (26), 4970-4982 DOI: 10.1038/s41388-020-1333-1
Cited by 39
AI Summary: OriGene’s pU6-ZSCAN4 shRNA knockdown vector was used in this study to silence ZSCAN4 expression and assess its role in maintaining cancer stem cell marker expression and phenotype.
Snippet: otency factors OCT3/4, NANOG, and SOX2 also mark CSCs [5–7]. To determine if ZSCAN4 is needed for the maintenance of CSC, and CSC marker expression, we first used pU6-ZSCAN4 shRNA knockdown vector (Origene) (containing RFP reporter gene and puromycin resistance gene) (Supplementary Fig. 6a). We then tested the knockdown efficiency of four ZSCAN4 shRNA sequences (named shRNA1–shRNA4) by transfe
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
