ZSCAN4 (NM_152677) Human Over-expression Lysate
SKU
LC407355
Lyophilized ZSCAN4 HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC211003 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_152677, NP_689890 |
| Synonyms | ZNF494 |
| Predicted MW | 49 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_689890 |
| Locus ID | 201516 |
| Cytogenetics | 19q13.43 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "ZSCAN4" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH311003 | ZSCAN4 MS Standard C13 and N15-labeled recombinant protein (NP_689890) | 10 ug |
$3,360.00
|
|
| LY407355 | Transient overexpression lysate of zinc finger and SCAN domain containing 4 (ZSCAN4) (as WB positive control) | 100 ug |
$436.00
|
|
| TP311003 | Recombinant protein of human zinc finger and SCAN domain containing 4 (ZSCAN4), 20 µg | 20 ug |
$737.00
|
|
Citations
ZSCAN4 facilitates chromatin remodeling and promotes the cancer stem cell phenotype
Portney, Arad, Gupta et al
Oncogene (2020) 39 (26), 4970-4982 DOI: 10.1038/s41388-020-1333-1
Portney, Arad, Gupta et al
Oncogene (2020) 39 (26), 4970-4982 DOI: 10.1038/s41388-020-1333-1
Cited by 39
AI Summary: OriGene’s pU6-ZSCAN4 shRNA knockdown vector was used in this study to silence ZSCAN4 expression and assess its role in maintaining cancer stem cell marker expression and phenotype.
Snippet: otency factors OCT3/4, NANOG, and SOX2 also mark CSCs [5–7]. To determine if ZSCAN4 is needed for the maintenance of CSC, and CSC marker expression, we first used pU6-ZSCAN4 shRNA knockdown vector (Origene) (containing RFP reporter gene and puromycin resistance gene) (Supplementary Fig. 6a). We then tested the knockdown efficiency of four ZSCAN4 shRNA sequences (named shRNA1–shRNA4) by transfe
Showing 1-1 of 1 papers.
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