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RAB23 (NM_183227) Human Over-expression Lysate

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SKU
LY405187
Transient overexpression lysate of RAB23, member RAS oncogene family (RAB23), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221508
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_183227, NP_899050
Synonyms HSPC137
Predicted MW 26.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_899050
Locus ID 51715
Cytogenetics 6p12.1-p11.2
Protein Families WB
Protein Pathways Hedgehog signaling pathway
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Other "RAB23" proteins (7)
SKU Description Size Price
PH301922 RAB23 MS Standard C13 and N15-labeled recombinant protein (NP_057361) 10 ug
$3,360.00
PH321508 RAB23 MS Standard C13 and N15-labeled recombinant protein (NP_899050) 10 ug
$3,360.00
LC402532 Lyophilized RAB23 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC405187 Lyophilized RAB23 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402532 Transient overexpression lysate of RAB23, member RAS oncogene family (RAB23), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP301922 Recombinant protein of human RAB23, member RAS oncogene family (RAB23), transcript variant 1, 20 µg 20 ug
$867.00
TP321508 Recombinant protein of human RAB23, member RAS oncogene family (RAB23), transcript variant 2, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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