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RAB5C (NM_201434) Human Over-expression Lysate

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SKU
LY404416
Transient overexpression lysate of RAB5C, member RAS oncogene family (RAB5C), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210698
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_201434, NP_958842
Synonyms L1880; RAB5CL; RAB5L; RABL
Predicted MW 23.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_958842
Locus ID 5878
Cytogenetics 17q21.2
Protein Families WB
Protein Pathways Endocytosis
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Other "RAB5C" proteins (3)
SKU Description Size Price
PH310698 RAB5C MS Standard C13 and N15-labeled recombinant protein (NP_958842) 10 ug
$3,360.00
LC404416 Lyophilized RAB5C HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310698 Purified recombinant protein of Homo sapiens RAB5C, member RAS oncogene family (RAB5C), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Endosomal Escape of ASOs Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation
Miller, Wan, Seth et al
Nucleic Acid Ther (2018)
AI Summary: OriGene’s Rab5 plasmid (NM_201434) was used for Rab5C overexpression in cell experiments to investigate its role in the endosomal trafficking and escape of antisense oligonucleotides.
Snippet: Rab5C overexpression and Western blot analysis The Rab5 plasmid (OriGene NM_201434) was transiently transfected into 190-kDa HARE-overexpressing cells using Lipofectamine 2000 under predetermined optimal conditions
Showing 1-1 of 1 papers.
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