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RAB5C (NM_201434) Human Recombinant Protein

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SKU
TP310698
Purified recombinant protein of Homo sapiens RAB5C, member RAS oncogene family (RAB5C), transcript variant 1, 20 µg
Size:
20 ug 1 mg 100 ug
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC210698 representing NM_201434
Red=Cloning site Green=Tags(s)

MAGRGGAARPNGPAAGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTV
KFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNTDTFARAKNWVKELQRQASPNIVIALAGNKADLAS
KRAVEFQEAQAYADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQNATGAPGRNRGVDLQENNPASR
SQCCSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 23.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity Enzyme activity (PMID: 26030178)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_958842
Locus ID 5878
UniProt ID P51148
Cytogenetics 17q21.2
RefSeq Size 1791
RefSeq ORF 648
Synonyms L1880; RAB5CL; RAB5L; RABL
Summary Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 PubMed 8646882).supplied by OMIM, Nov 2010
Protein Categories Intracellular Proteins
Protein Families Druggable Genome
Protein Pathways Endocytosis
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Other "RAB5C" proteins (3)
SKU Description Size Price
PH310698 RAB5C MS Standard C13 and N15-labeled recombinant protein (NP_958842) 10 ug
$3,360.00
LC404416 Lyophilized RAB5C HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404416 Transient overexpression lysate of RAB5C, member RAS oncogene family (RAB5C), transcript variant 1 (as WB positive control) 100 ug
$436.00

file-alt Citations

Epigenetic activation of a cryptic TBC1D16 transcript enhances melanoma progression by targeting EGFR
Vizoso, Ferreira, Lopez-Serra et al
Nat. Med. (2015)
Cited by 120
AI Summary: In this study, OriGene’s recombinant RAB5C GTPase (catalog number TP310698) was used to investigate the interaction between TBC1D16-47KD and RAB5C, helping to elucidate the molecular mechanisms underlying melanoma progression.
Snippet: FLAG-TBC1D16-47KD protein was purified from HEK293T cells. Recombinant RAB5C GTPase (100 pmol) (Origene, TP310698) was loaded with [γ−32P]GTP for 10 min at 25 °C and desalted on PD-SpinTrapTM G-25 columns (GE Healthcare, # 28-9180-04) to remove free nucleotide
Showing 1-1 of 1 papers.
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