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CD59 (NM_203329) Human Over-expression Lysate

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SKU
LY404397
Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 3 (as WB positive control)
  • LY404397 is the same product as LY430908.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
In Stock*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200736
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_203329, NP_976074
Synonyms 1F5; 16.3A5; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20
Predicted MW 14.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_976074
Locus ID 966
Cytogenetics 11p13
Protein Families WB
Protein Pathways Complement and coagulation cascades, Hematopoietic cell lineage
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Other "CD59" proteins (23)
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PH318343 CD59 MS Standard C13 and N15-labeled recombinant protein (NP_000602) 10 ug
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LC404397 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404398 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404399 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC424608 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC426723 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426724 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426725 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426726 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430908 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430909 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430910 Lyophilized CD59 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404398 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY404399 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY424608 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY426723 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 5 (as WB positive control) 100 ug
$436.00
LY426724 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 6 (as WB positive control) 100 ug
$436.00
LY426725 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 7 (as WB positive control) 100 ug
$436.00
LY426726 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 8 (as WB positive control) 100 ug
$436.00
LY430908 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY430909 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY430910 Transient overexpression lysate of CD59 molecule, complement regulatory protein (CD59), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP318343 Recombinant protein of human CD59 molecule, complement regulatory protein (CD59), transcript variant 2, 20 µg 20 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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