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BDH2 (NM_020139) Human Over-expression Lysate

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SKU
LY402755
Transient overexpression lysate of 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210586
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_020139, NP_064524
Synonyms DHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308
Predicted MW 26.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_064524
Locus ID 56898
Cytogenetics 4q24
Protein Families WB
Protein Pathways Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies
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Other "BDH2" proteins (4)
SKU Description Size Price
PH310586 BDH2 MS Standard C13 and N15-labeled recombinant protein (NP_064524) 10 ug
$3,360.00
LC402755 Lyophilized BDH2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310586 Recombinant protein of human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2), 20 µg 20 ug
$867.00
TP720509 Recombinant protein of human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) 10 ug
$340.00

file-alt Citations

Direct detection of alternative open reading frames translation products in human significantly expands the proteome
Vanderperre, Lucier, Bissonnette et al
PLoS ONE (2013) 8 (8), e70698 DOI: 10.1371/journal.pone.0070698
Cited by 223
AI Summary: OriGene’s cDNA plasmid clone SC119794 was used to PCR amplify BDRKB2 in this study, enabling the investigation of alternative open reading frame translation products and their contribution to the expanded human proteome.
Snippet: 1A). Reference proteins populate current protein databases used to support research in life sciences. ... PCR fragments were inserted into the BamHI site of pcDNA HA-EGFP+2 . BDRKB2 was PCR amplified from a cDNA containing plasmid (clone SC119794, OriGene), and the
FigureFigureFigure
Showing 1-1 of 1 papers.
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