BDH2 (NM_020139) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BDH2 |
| Synonyms | DHRS6; EFA6R; PRO20933; SDR15C1; UCPA-OR; UNQ6308 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020139 |
| ORF Size | 735 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020139.4 |
| RefSeq Size | 2936 bp |
| RefSeq ORF | 738 bp |
| Locus ID | 56898 |
| UniProt ID | Q9BUT1 |
| Cytogenetics | 4q24 |
| Domains | adh_short |
| Protein Families | Druggable Genome |
| Protein Pathways | Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies |
| MW | 26.8 kDa |
| Summary | Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron assimilation and homeostasis. Plays a role in susceptibility to bacterial infection by providing an assimilable source of iron that is exploited by pathogenic bacteria (By similarity). Also acts as a 3-hydroxybutyrate dehydrogenase (PubMed:16380372).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020139" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210586L3 | Lenti ORF clone of Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210586L4 | Lenti ORF clone of Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2), mGFP tagged | 10 ug |
$600.00
|
|
| RG210586 | BDH2 (tGFP-tagged) - Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) | 10 ug |
$489.00
$500.00
|
|
| SC113190 | BDH2 (untagged)-Human 3-hydroxybutyrate dehydrogenase, type 2 (BDH2) | 10 ug |
$300.00
|
|
Citations
Dysregulation of Ketone Body Metabolism Is Associated With Poor Prognosis for Clear Cell Renal Cell Carcinoma Patients
Cui, Luo, Zhou et al
Front Oncol (2019) 9, 1422 DOI: 10.3389/fonc.2019.01422
Cui, Luo, Zhou et al
Front Oncol (2019) 9, 1422 DOI: 10.3389/fonc.2019.01422
Cited by 31
AI Summary: In this study, OriGene’s ACAT1 (RC200844), BDH2 (RC202707), and HMGCL (RC217739) cDNA clones were used to ectopically express these genes in ccRCC cells, enabling the researchers to demonstrate their tumor-suppressive effects and investigate the role of ketone body metabolism in clear cell renal cell carcinoma.
Inactivation of 3-hydroxybutyrate dehydrogenase type 2 promotes proliferation and metastasis of nasopharyngeal carcinoma by iron retention
Li, Liao, Mo et al
Br. J. Cancer (2019) 122 (1), 102-110 DOI: 10.1038/s41416-019-0638-8
Li, Liao, Mo et al
Br. J. Cancer (2019) 122 (1), 102-110 DOI: 10.1038/s41416-019-0638-8
Cited by 26
AI Summary: OriGene’s BDH2 plasmid (subcloned into the pCMV6-Entry vector) was used to restore BDH2 expression in nasopharyngeal carcinoma cells, enabling the study of its role in cell proliferation, migration, and iron metabolism.
Snippet: BDH2 plasmid was purchased from Origene (Rockville, MD, USA);the open-reading frame of BDH2 was subcloned into the pCMV6-Entry vector. The protocol of transfection has been described. 20. RNA extraction, cDNA synthesis and real-time PCR;- BDH2 plasmid was purchased from Origene (Rockville, MD, USA)
Downregulation of BDH2 modulates iron homeostasis and promotes DNA demethylation in CD4+ T cells of systemic lupus erythematosus
Zhao, Li, Gao et al
Clin. Immunol. (2017)
Zhao, Li, Gao et al
Clin. Immunol. (2017)
Cited by 69
Showing 1-3 of 3 papers.
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