E Cadherin (CDH1) (NM_004360) Human Over-expression Lysate
SKU
LY401389
Transient overexpression lysate of cadherin 1, type 1, E-cadherin (epithelial) (CDH1) (as WB positive control)
$665.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC220731 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_004360, NP_004351 |
| Synonyms | Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO |
| Predicted MW | 97.46 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_004351 |
| Locus ID | 999 |
| Cytogenetics | 16q22.1 |
| Protein Families | WB |
| Protein Pathways | Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "E Cadherin" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH320731 | CDH1 MS Standard C13 and N15-labeled recombinant protein (NP_004351) | 10 ug |
$3,360.00
|
|
| LC401389 | Lyophilized CDH1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP320731 | Recombinant protein of human cadherin 1, type 1, E-cadherin (epithelial) (CDH1), 20 µg | 20 ug |
$737.00
|
|
Citations
Downregulation of E-cadherin is an essential event in activating beta-catenin/Tcf-dependent transcription and expression of its target genes in Pdcd4 knockdown cells
Wang, Sun, Allgayer et al
Oncogene (2010) 29 (1), 128-38 DOI: 10.1038/onc.2009.302
Wang, Sun, Allgayer et al
Oncogene (2010) 29 (1), 128-38 DOI: 10.1038/onc.2009.302
Cited by 89
AI Summary: OriGene products contributed to this study by providing the E-cadherin expression plasmid (RC208446) and E-cadherin shRNA (TF314697), which were used to manipulate E-cadherin levels and assess its role in beta-catenin/Tcf-dependent transcription and tumor cell invasion.
Showing 1-1 of 1 papers.
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