E Cadherin (CDH1) (NM_004360) Human Tagged ORF Clone
$808.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | E Cadherin |
| Synonyms | Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004360 |
| ORF Size | 2646 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004360.5 |
| RefSeq Size | 4828 bp |
| RefSeq ORF | 2649 bp |
| Locus ID | 999 |
| UniProt ID | P12830, A0A0U2ZQU7, B3GN61 |
| Cytogenetics | 16q22.1 |
| Domains | CA, Cadherin_C_term |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer |
| MW | 97.46 kDa |
| Summary | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. provided by RefSeq, Nov 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004360" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC220731L1 | Lenti-ORF clone of CDH1 (Myc-DDK-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) | 10 ug |
$1,108.00
|
|
| RC220731L2 | Lenti-ORF clone of CDH1 (mGFP-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) | 10 ug |
$1,108.00
|
|
| RC220731L3 | Lenti-ORF clone of CDH1 (Myc-DDK-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) | 10 ug |
$1,108.00
|
|
| RC220731L4 | Lenti-ORF clone of CDH1 (mGFP-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) | 10 ug |
$1,108.00
|
|
| RG220731 | CDH1 (tGFP-tagged) - Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) | 10 ug |
$789.00
$1,008.00
|
|
| SC117413 | CDH1 (untagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) | 10 ug |
$1,373.00
|
|
Citations
Induced Tamoxifen Resistance is Mediated by Increased Methylation of E-Cadherin in Estrogen Receptor-Expressing Breast Cancer Cells
Wang, Gun, Hong
Sci Rep (2019) 9 (1), 14140 DOI: 10.1038/s41598-019-50749-1
Wang, Gun, Hong
Sci Rep (2019) 9 (1), 14140 DOI: 10.1038/s41598-019-50749-1
AI Summary: OriGene contributed to this study by providing the E-cadherin expression plasmid (E-cadherin cloned in pCMV6), which was used to overexpress E-cadherin in breast cancer cells to investigate its role in tamoxifen resistance.
Snippet: Rad, China). The mRNA expression levels of were quantified by measuring the threshold cycle (Ct). E-cadherin over-expression. E-cadherin, cloned in pCMV6, was obtained from Origene (Beijing, China). Transfection of cloned
miR-25 promotes invasion of human non-small cell lung cancer via CDH1
Liu, Sun
Bioengineered (2019) 10 (1), 271-281 DOI: 10.1080/21655979.2019.1632668
Liu, Sun
Bioengineered (2019) 10 (1), 271-281 DOI: 10.1080/21655979.2019.1632668
AI Summary: In this study, the pCMV6-CDH1 plasmid from OriGene Technologies was used to restore CDH1 expression and investigate its role in miR-25-mediated invasion of non-small cell lung cancer cells.
Snippet: were stained by 0.5% crystal violet dissolved in 100% methanol. pCMV6- CDH1, which contains CDH1 open reading frame was purchased from OriGene Technologies. To inhibit miR-25 expression, Calu-1 cells were plated
MiR-23a promotes TGF-β1-induced EMT and tumor metastasis in breast cancer cells by directly targeting CDH1 and activating Wnt/β-catenin signaling
Ma, Li, Liu et al
Oncotarget (2017) 8 (41), 69538-69550 DOI: 10.18632/oncotarget.18422
Ma, Li, Liu et al
Oncotarget (2017) 8 (41), 69538-69550 DOI: 10.18632/oncotarget.18422
Snippet: MiR-23a mimics, scrambled miR-control, CDH1 siRNA or siRNA negative control, purchased from GenePharma (China), were transfected into 70% of the indicated cells according to the manufacturer’s protocol. CDH1 Human cDNA ORF Clone was purchased from Origene (Origene Technologies)
Overexpression of CASS4 promotes invasion in non-small cell lung cancer by activating the AKT signaling pathway and inhibiting E-cadherin expression
Li, Zhang, Xia et al
Tumour Biol. (2016)
Li, Zhang, Xia et al
Tumour Biol. (2016)
Cited by 10
Snippet: loading control. Plasmid transfection Empty pCMV6-Entry vector, pCMV6-Entry-CASS4 plas- mid, and pCMV6-Entry-E-cadherin were purchased from OriGene Technologies (Rockville, MD, USA). Plasmids were introduced ... Cite SaveSaving
microRNA-92a promotes lymph node metastasis of human esophageal squamous cell carcinoma via E-cadherin
Chen, Zhao, Wang et al
J. Biol. Chem. (2011) 286 (12), 10725-34 DOI: 10.1074/jbc.M110.165654
Chen, Zhao, Wang et al
J. Biol. Chem. (2011) 286 (12), 10725-34 DOI: 10.1074/jbc.M110.165654
Cited by 50
AI Summary: OriGene’s pCMV6-ENTRY-CDH1 plasmid, containing the CDH1 open reading frame without the 3′UTR, was used to restore CDH1 expression in ESCC cells and investigate the role of CDH1 suppression in miR-92a-mediated metastasis.
Snippet: pCMV6-ENTRY-CDH1, which contains CDH1 open reading frame without 3′UTR, was purchased from OriGene Technologies. ... 2C). However, overexpression of miR-92a in KYSE410 cells and knock-down of miR-92a in EC9706 cells had no significant effect on cell apoptosis
Showing 1-5 of 5 papers.
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