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E Cadherin (CDH1) (NM_004360) Human Untagged Clone

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SKU
SC117413
CDH1 (untagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1)
  $1,373.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol E Cadherin
Synonyms Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_004360, the custom clone sequence may differ by one or more nucleotides


ACCAGGCTTGCGGAAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGCACCCGGCGC
CTGCCCTCGCTCGGCGTCCCCGGCCAGCCATGGGCCCTTGGAGCCGCAGCCTCTCGGCGCTGCTGCTGCT
GCTGCAGGTCTCCTCTTGGCTCTGCCAGGAGCCGGAGCCCTGCCACCCTGGCTTTGACGCCGAGAGCTAC
ACGTTCACGGTGCCCCGGCGCCACCTGGAGAGAGGCCGCGTCCTGGGCAGAGTGAATTTTGAAGATTGCA
CCGGTCGACAAAGGACAGCCTATTTTTCCCTCGACACCCGATTCAAAGTGGGCACAGATGGTGTGATTAC
AGTCAAAAGGCCTCTACGGTTTCATAACCCACAGATCCATTTCTTGGTCTACGCCTGGGACTCCACCTAC
AGAAAGTTTTCCACCAAAGTCACGCTGAATACAGTGGGGCACCACCACCGCCCCCCGCCCCATCAGGCCT
CCGTTTCTGGAATCCAAGCAGAATTGCTCACATTTCCCAACTCCTCTCCTGGCCTCAGAAGACAGAAGAG
AGACTGGGTTATTCCTCCCATCAGCTGCCCAGAAAATGAAAAAGGCCCATTTCCTAAAAACCTGGTTCAG
ATCAAATCCAACAAAGACAAAGAAGGCAAGGTTTTCTACAGCATCACTGGCCAAGGAGCTGACACACCCC
CTGTTGGTGTCTTTATTATTGAAAGAGAAACAGGATGGCTGAAGGTGACAGAGCCTCTGGATAGAGAACG
CATTGCCACATACACTCTCTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGGAG
ATTTTGATCACGGTAACCGATCAGAATGACAACAAGCCCGAATTCACCCAGGAGGTCTTTAAGGGGTCTG
TCATGGAAGGTGCTCTTCCAGGAACCTCTGTGATGGAGGTCACAGCCACAGACGCGGACGATGATGTGAA
CACCTACAATGCCGCCATCGCTTACACCATCCTCAGCCAAGATCCTGAGCTCCCTGACAAAAATATGTTC
ACCATTAACAGGAACACAGGAGTCATCAGTGTGGTCACCACTGGGCTGGACCGAGAGAGTTTCCCTACGT
ATACCCTGGTGGTTCAAGCTGCTGACCTTCAAGGTGAGGGGTTAAGCACAACAGCAACAGCTGTGATCAC
AGTCACTGACACCAACGATAATCCTCCGATCTTCAATCCCACCACGTACAAGGGTCAGGTGCCTGAGAAC
GAGGCTAACGTCGTAATCACCACACTGAAAGTGACTGATGCTGATGCCCCCAATACCCCAGCGTGGGAGG
CTGTATACACCATATTGAATGATGATGGTGGACAATTTGTCGTCACCACAAATCCAGTGAACAACGATGG
CATTTTGAAAACAGCAAAGGGCTTGGATTTTGAGGCCAAGCAGCAGTACATTCTACACGTAGCAGTGACG
AATGTGGTACCTTTTGAGGTCTCTCTCACCACCTCCACAGCCACCGTCACCGTGGATGTGCTGGATGTGA
ATGAAGCCCCCATCTTTGTGCCTCCTGAAAAGAGAGTGGAAGTGTCCGAGGACTTTGGCGTGGGCCAGGA
AATCACATCCTACACTGCCCAGGAGCCAGACACATTTATGGAACAGAAAATAACATATCGGATTTGGAGA
GACACTGCCAACTGGCTGGAGATTAATCCGGACACTGGTGCCATTTCCACTCGGGCTGAGCTGGACAGGG
AGGATTTTGAGCACGTGAAGAACAGCACGTACACAGCCCTAATCATAGCTACAGACAATGGTTCTCCAGT
TGCTACTGGAACAGGGACACTTCTGCTGATCCTGTCTGATGTGAATGACAACGCCCCCATACCAGAACCT
CGAACTATATTCTTCTGTGAGAGGAATCCAAAGCCTCAGGTCATAAACATCATTGATGCAGACCTTCCTC
CCAATACATCTCCCTTCACAGCAGAACTAACACACGGGGCGAGTGCCAACTGGACCATTCAGTACAACGA
CCCAACCCAAGAATCTATCATTTTGAAGCCAAAGATGGCCTTAGAGGTGGGTGACTACAAAATCAATCTC
AAGCTCATGGATAACCAGAATAAAGACCAAGTGACCACCTTAGAGGTCAGCGTGTGTGACTGTGAAGGGG
CCGCCGGCGTCTGTAGGAAGGCACAGCCTGTCGAAGCAGGATTGCAAATTCCTGCCATTCTGGGGATTCT
TGGAGGAATTCTTGCTTTGCTAATTCTGATTCTGCTGCTCTTGCTGTTTCTTCGGAGGAGAGCGGTGGTC
AAAGAGCCCTTACTGCCCCCAGAGGATGACACCCGGGACAATGTTTATTACTATGATGAAGAAGGAGGCG
GAGAAGAGGACCAGGACTTTGACTTGAGCCAGCTGCACAGGGGCCTGGACGCTCGGCCTGAAGTGACTCG
TAACGACGTTGCACCAACCCTCATGAGTGTCCCCCGGTATCTTCCCCGCCCTGCCAATCCCGATGAAATT
GGAAATTTTATTGATGAAAATCTGAAAGCGGCTGATACTGACCCCACAGCCCCGCCTTATGATTCTCTGC
TCGTGTTTGACTATGAAGGAAGCGGTTCCGAAGCTGCTAGTCTGAGCTCCCTGAACTCCTCAGAGTCAGA
CAAAGACCAGGACTATGACTACTTGAACGAATGGGGCAATCGCTTCAAGAAGCTGGCTGACATGTACGGA
GGCGGCGAGGACGACTAGGGGACTCGAGAGAGGCGGGCCCCAGACCCATGTGCTGGGAAATGCAGAAATC
ATGTTGCTGGTGGTTTTTCAGCTCCCTTCCCTTGAGATGAGTTTCTGG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_004360 unedited
GGGTTGCAGNATTTTGTAATACGATTCACTATAGGCGGCCCGCGAATCGGCACCAGGCTT
GCGGTAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGCACCCGGCG
CCTGCCCTCGCTCGGCGTCCCCGGCCAGCCATGGGCCCTTGGAGCCGCAGCCTCTCGGCG
CTGCTGCTGCTGCTGCAGGTCTCCTCTTGGCTCTGCCAGGAGCCGGAGCCCTGCCACCCT
GGCTTTGACGCCGAGAGCTACACGTTCACGGTGCCCCGGCGCCACCTGGAGAGAGGCCGC
GTCCTGGGCAGAGTGAATTTTGAAGATTGCACCGGTCGACAAAGGACAGCCTATTTTTCC
CTCGACACCCGATTCAAAGTGGGCACAGATGGTGTGATTACAGTCAAAAGGCCTCTACGG
TTTCATAACCCACAGATCCATTTCTTGGTCTACGCCTGGGACTCCACCTACAGAAAGTTT
TCCACCAAAGTCACGCTGAATACAGTGGGGCACCACCACCGCCCCCCGCCCCATCAGGCC
TCCGTTTCTGGAATCCAAGCAGAATTGCTCACATTTCCCAACTCCTCTCCTGGCCTCAGA
AGACAGAAGAGAGACTGGGTTATTCCTCCCATCAGCTGCCCAGAAAATGAAAAAGGCCCA
TTTCCTAAAAACCTGGTTCAGATCAAATCCAACAAAGACAAAGAAGGCAAGGTTTTCTAC
AGCATCACTGGCCAAGGAGCTGACACACCCCCTGTTGGTGTCTTTATTATTTGAAAGAGA
ACAGTATGGCTGAAGGTGACAGAGCCTCTGCATAGAGAACGCACTGCCCCATACACTCTC
TTTTCTCACGCTGTGTCATCCAACGGAAATGCCGTTGAGGATCCATGGAGAATTTGATCA
CGGTACCGATCAAATGACAACAGCCCGAATCACCG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_004360 unedited
TATGGACCGCGGCCGCATCTAAATCGAGTTTTTTTTTTTTTTTTTTAATGGTTTAACAAA
ATTGTTTAATAAAATTTATAAAAATGCATCTTTGAGAATACTTTTCTCAGCTTGAATTGT
TTTCCTTTTCCACCCCCAAAGAAAATACACAATTATCAGCACCCACACATGTATACACTC
AAAACTACAGTGACATTCTCTACACAGAACTATATTCGATATAGCTTGAACTGCCGAAAA
ATCAAGACAATTCCAAAAAGTGATTGCAGGGTTGATTTTTTTCTCCAAAACACTCGAGAA
ACAGTAAAGCTATTTGAACAAAAGACTTTTGTTTGATTGCCAAAAGTCGAAATCCACATT
TAAATGTACCGAGATGCAACGTCCGATGCCCGCTACCCTTTTTCCCCGCCCCACCCCCGT
TTTTGGACCCACCCTCTTCACCGTTTTCCCCCCCTTTGTCCTTTTTTTCTTTTGCCTCCC
CCCCCCCCCCCCTTTTTCCCCCGCTCCAGCCTAGTTCGCCCGCATATCCCCACCCCGCCC
CGCCCATTGTCTCCCGCCTTGTGTGCCCAGCTGCCACCGCCCTACCCCCACTCCCCCGCG
TATCTGCCAGATGTTGTTCGCGCGGCGGGGCGTCGCGGGGAGGTGCGCCCCCCGCCCCTC
CATTCTGTCGCCGGTTTGCAGTCCCTCTTGTGTGCTTTTTTAGCGCGGGTGGCCTCCGTG
GGGCAACCCGCCCACCCGTCCGCCCCTCTTTGTCTCCTCCAGTCTTGTACATAATCTCCA
TAGTATGCCCTGAAGTCGTCGCTCCCACCCCCCCGCACCCATTGCGCCCCCTCGCGGTTC
TCCCGCCTCATCTTGCCCCACTCCGTCGTCCCGTCGCGTTTCTCAACCAGTGGCGTTCTC
ACCCCGCATCATTTCACCGCCCCCTCTTGTCCCAGCCCCCTTTGCTCCACCGCGGTTACG
TCAGACCCTCACGTATTCCGCCGTCCGGGCCCTCCCGCTAGCCCACTTGCCGCCCTACG
Restriction Sites NotI-NotI |
ACCN NM_004360
Insert Size 5000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004360.2, NP_004351.1
RefSeq Size 4815 bp
RefSeq ORF 4815 bp
Locus ID 999
UniProt ID P12830
Cytogenetics 16q22.1
Domains CA, Cadherin_C_term
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer
Summary This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. provided by RefSeq, Nov 2015
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
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"NM_004360" in other vectors (6)
SKU Description Size Price
RC220731 CDH1 (Myc-DDK-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) 10 ug
$808.00
RC220731L1 Lenti-ORF clone of CDH1 (Myc-DDK-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) 10 ug
$1,108.00
RC220731L2 Lenti-ORF clone of CDH1 (mGFP-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) 10 ug
$1,108.00
RC220731L3 Lenti-ORF clone of CDH1 (Myc-DDK-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) 10 ug
$1,108.00
RC220731L4 Lenti-ORF clone of CDH1 (mGFP-tagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) 10 ug
$1,108.00
RG220731 CDH1 (tGFP-tagged) - Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) 10 ug
$789.00 $1,008.00

file-alt Citations

Molecular and proteomic insight into Notch1 characterization in hepatocellular carcinoma
Giovannini, Minguzzi, Genovese et al
Oncotarget (2016) 7 (26), 39609-39626 DOI: 10.18632/oncotarget.9203
Snippet: For plasmid transfection SNU398 cells were seeded into 6 well plates and transfected with 0,2 μg of pCMV6-XL4-CDH1 plasmid, containing the full length human CDH1 cDNA, or empty vector pCMV6-XL4 (OriGene Technologies, Rockville, MD) using Lipofectamine 2000
FigureFigureFigure
Nuclear factor I-C regulates E-cadherin via control of KLF4 in breast cancer
Lee, Lee, Park
BMC Cancer (2015) 15, 113 DOI: 10.1186/s12885-015-1118-z
Cited by 51
AI Summary: OriGene products contributed to this study by providing the human NFI-C expression plasmid (RC209307) and NFI-C shRNA (TF311647), which were used to manipulate NFI-C levels in breast cancer cells to investigate its role in regulating KLF4 and E-cadherin expression.
FigureFigureFigure
Nuclear factor I-C (NFIC) Regulates Dentin Sialophosphoprotein (DSPP) and E-cadherin via Control of Kruppel-like factor 4 (KLF4) during Dentinogenesis
Lee, Lee, Park et al
J. Biol. Chem. (2014)
AI Summary: OriGene products, including the pcDNA3-Flag-Slug, pGL2-Klf4, pGL2-Slug, and pGL2-E-cadherin plasmids, were used in this study to investigate the regulatory roles of NFIC and KLF4 in odontoblast differentiation and dentinogenesis.
Snippet: The pcDNA3-Flag-Slug, pGL2-Klf4, -Slug, and -E-cadherin plasmids were purchased from Origene Company (Rockville, MD). Deletion mutants of the Klf4 promoter regions were generated by PCR amplification and subcloned into pGL3 (Promega, Madison, WI)
Epigenetic loss of CDH1 correlates with multidrug resistance in human hepatocellular carcinoma cells
Jiang, Chan, Fung
Biochem. Biophys. Res. Commun. (2012) 422 (4), 739-44 DOI: 10.1016/j.bbrc.2012.05.072
Cited by 17
AI Summary: OriGene's pCMV6-XL4-CDH1 plasmid, containing the full-length CDH1 cDNA, was used to restore CDH1 expression in multidrug-resistant hepatocellular carcinoma cells, enabling the study of CDH1's role in drug resistance.
Snippet: It was established in our laboratory by long-term culturing HepG2 cell with increasing concentrations of doxorubicin and found to be ... containing the full length cDNA of CDH1 in the vector pCMV6-XL4 (pCMV6-XL4-CDH1) was purchased from Origene Technologies Incoporation
Showing 1-5 of 5 papers.
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