E Cadherin (CDH1) (NM_004360) Human Untagged Clone

SKU SC117413

CDH1 (untagged)-Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: E Cadherin
• Synonyms: Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004360, the custom clone sequence may differ by one or more nucleotides; ; ; ACCAGGCTTGCGGAAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGCACCCGGCGC; CTGCCCTCGCTCGGCGTCCCCGGCCAGCCATGGGCCCTTGGAGCCGCAGCCTCTCGGCGCTGCTGCTGCT; GCTGCAGGTCTCCTCTTGGCTCTGCCAGGAGCCGGAGCCCTGCCACCCTGGCTTTGACGCCGAGAGCTAC; ACGTTCACGGTGCCCCGGCGCCACCTGGAGAGAGGCCGCGTCCTGGGCAGAGTGAATTTTGAAGATTGCA; CCGGTCGACAAAGGACAGCCTATTTTTCCCTCGACACCCGATTCAAAGTGGGCACAGATGGTGTGATTAC; AGTCAAAAGGCCTCTACGGTTTCATAACCCACAGATCCATTTCTTGGTCTACGCCTGGGACTCCACCTAC; AGAAAGTTTTCCACCAAAGTCACGCTGAATACAGTGGGGCACCACCACCGCCCCCCGCCCCATCAGGCCT; CCGTTTCTGGAATCCAAGCAGAATTGCTCACATTTCCCAACTCCTCTCCTGGCCTCAGAAGACAGAAGAG; AGACTGGGTTATTCCTCCCATCAGCTGCCCAGAAAATGAAAAAGGCCCATTTCCTAAAAACCTGGTTCAG; ATCAAATCCAACAAAGACAAAGAAGGCAAGGTTTTCTACAGCATCACTGGCCAAGGAGCTGACACACCCC; CTGTTGGTGTCTTTATTATTGAAAGAGAAACAGGATGGCTGAAGGTGACAGAGCCTCTGGATAGAGAACG; CATTGCCACATACACTCTCTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGGAG; ATTTTGATCACGGTAACCGATCAGAATGACAACAAGCCCGAATTCACCCAGGAGGTCTTTAAGGGGTCTG; TCATGGAAGGTGCTCTTCCAGGAACCTCTGTGATGGAGGTCACAGCCACAGACGCGGACGATGATGTGAA; CACCTACAATGCCGCCATCGCTTACACCATCCTCAGCCAAGATCCTGAGCTCCCTGACAAAAATATGTTC; ACCATTAACAGGAACACAGGAGTCATCAGTGTGGTCACCACTGGGCTGGACCGAGAGAGTTTCCCTACGT; ATACCCTGGTGGTTCAAGCTGCTGACCTTCAAGGTGAGGGGTTAAGCACAACAGCAACAGCTGTGATCAC; AGTCACTGACACCAACGATAATCCTCCGATCTTCAATCCCACCACGTACAAGGGTCAGGTGCCTGAGAAC; GAGGCTAACGTCGTAATCACCACACTGAAAGTGACTGATGCTGATGCCCCCAATACCCCAGCGTGGGAGG; CTGTATACACCATATTGAATGATGATGGTGGACAATTTGTCGTCACCACAAATCCAGTGAACAACGATGG; CATTTTGAAAACAGCAAAGGGCTTGGATTTTGAGGCCAAGCAGCAGTACATTCTACACGTAGCAGTGACG; AATGTGGTACCTTTTGAGGTCTCTCTCACCACCTCCACAGCCACCGTCACCGTGGATGTGCTGGATGTGA; ATGAAGCCCCCATCTTTGTGCCTCCTGAAAAGAGAGTGGAAGTGTCCGAGGACTTTGGCGTGGGCCAGGA; AATCACATCCTACACTGCCCAGGAGCCAGACACATTTATGGAACAGAAAATAACATATCGGATTTGGAGA; GACACTGCCAACTGGCTGGAGATTAATCCGGACACTGGTGCCATTTCCACTCGGGCTGAGCTGGACAGGG; AGGATTTTGAGCACGTGAAGAACAGCACGTACACAGCCCTAATCATAGCTACAGACAATGGTTCTCCAGT; TGCTACTGGAACAGGGACACTTCTGCTGATCCTGTCTGATGTGAATGACAACGCCCCCATACCAGAACCT; CGAACTATATTCTTCTGTGAGAGGAATCCAAAGCCTCAGGTCATAAACATCATTGATGCAGACCTTCCTC; CCAATACATCTCCCTTCACAGCAGAACTAACACACGGGGCGAGTGCCAACTGGACCATTCAGTACAACGA; CCCAACCCAAGAATCTATCATTTTGAAGCCAAAGATGGCCTTAGAGGTGGGTGACTACAAAATCAATCTC; AAGCTCATGGATAACCAGAATAAAGACCAAGTGACCACCTTAGAGGTCAGCGTGTGTGACTGTGAAGGGG; CCGCCGGCGTCTGTAGGAAGGCACAGCCTGTCGAAGCAGGATTGCAAATTCCTGCCATTCTGGGGATTCT; TGGAGGAATTCTTGCTTTGCTAATTCTGATTCTGCTGCTCTTGCTGTTTCTTCGGAGGAGAGCGGTGGTC; AAAGAGCCCTTACTGCCCCCAGAGGATGACACCCGGGACAATGTTTATTACTATGATGAAGAAGGAGGCG; GAGAAGAGGACCAGGACTTTGACTTGAGCCAGCTGCACAGGGGCCTGGACGCTCGGCCTGAAGTGACTCG; TAACGACGTTGCACCAACCCTCATGAGTGTCCCCCGGTATCTTCCCCGCCCTGCCAATCCCGATGAAATT; GGAAATTTTATTGATGAAAATCTGAAAGCGGCTGATACTGACCCCACAGCCCCGCCTTATGATTCTCTGC; TCGTGTTTGACTATGAAGGAAGCGGTTCCGAAGCTGCTAGTCTGAGCTCCCTGAACTCCTCAGAGTCAGA; CAAAGACCAGGACTATGACTACTTGAACGAATGGGGCAATCGCTTCAAGAAGCTGGCTGACATGTACGGA; GGCGGCGAGGACGACTAGGGGACTCGAGAGAGGCGGGCCCCAGACCCATGTGCTGGGAAATGCAGAAATC; ATGTTGCTGGTGGTTTTTCAGCTCCCTTCCCTTGAGATGAGTTTCTGG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004360 unedited; GGGTTGCAGNATTTTGTAATACGATTCACTATAGGCGGCCCGCGAATCGGCACCAGGCTT; GCGGTAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGCACCCGGCG; CCTGCCCTCGCTCGGCGTCCCCGGCCAGCCATGGGCCCTTGGAGCCGCAGCCTCTCGGCG; CTGCTGCTGCTGCTGCAGGTCTCCTCTTGGCTCTGCCAGGAGCCGGAGCCCTGCCACCCT; GGCTTTGACGCCGAGAGCTACACGTTCACGGTGCCCCGGCGCCACCTGGAGAGAGGCCGC; GTCCTGGGCAGAGTGAATTTTGAAGATTGCACCGGTCGACAAAGGACAGCCTATTTTTCC; CTCGACACCCGATTCAAAGTGGGCACAGATGGTGTGATTACAGTCAAAAGGCCTCTACGG; TTTCATAACCCACAGATCCATTTCTTGGTCTACGCCTGGGACTCCACCTACAGAAAGTTT; TCCACCAAAGTCACGCTGAATACAGTGGGGCACCACCACCGCCCCCCGCCCCATCAGGCC; TCCGTTTCTGGAATCCAAGCAGAATTGCTCACATTTCCCAACTCCTCTCCTGGCCTCAGA; AGACAGAAGAGAGACTGGGTTATTCCTCCCATCAGCTGCCCAGAAAATGAAAAAGGCCCA; TTTCCTAAAAACCTGGTTCAGATCAAATCCAACAAAGACAAAGAAGGCAAGGTTTTCTAC; AGCATCACTGGCCAAGGAGCTGACACACCCCCTGTTGGTGTCTTTATTATTTGAAAGAGA; ACAGTATGGCTGAAGGTGACAGAGCCTCTGCATAGAGAACGCACTGCCCCATACACTCTC; TTTTCTCACGCTGTGTCATCCAACGGAAATGCCGTTGAGGATCCATGGAGAATTTGATCA; CGGTACCGATCAAATGACAACAGCCCGAATCACCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004360 unedited; TATGGACCGCGGCCGCATCTAAATCGAGTTTTTTTTTTTTTTTTTTAATGGTTTAACAAA; ATTGTTTAATAAAATTTATAAAAATGCATCTTTGAGAATACTTTTCTCAGCTTGAATTGT; TTTCCTTTTCCACCCCCAAAGAAAATACACAATTATCAGCACCCACACATGTATACACTC; AAAACTACAGTGACATTCTCTACACAGAACTATATTCGATATAGCTTGAACTGCCGAAAA; ATCAAGACAATTCCAAAAAGTGATTGCAGGGTTGATTTTTTTCTCCAAAACACTCGAGAA; ACAGTAAAGCTATTTGAACAAAAGACTTTTGTTTGATTGCCAAAAGTCGAAATCCACATT; TAAATGTACCGAGATGCAACGTCCGATGCCCGCTACCCTTTTTCCCCGCCCCACCCCCGT; TTTTGGACCCACCCTCTTCACCGTTTTCCCCCCCTTTGTCCTTTTTTTCTTTTGCCTCCC; CCCCCCCCCCCCTTTTTCCCCCGCTCCAGCCTAGTTCGCCCGCATATCCCCACCCCGCCC; CGCCCATTGTCTCCCGCCTTGTGTGCCCAGCTGCCACCGCCCTACCCCCACTCCCCCGCG; TATCTGCCAGATGTTGTTCGCGCGGCGGGGCGTCGCGGGGAGGTGCGCCCCCCGCCCCTC; CATTCTGTCGCCGGTTTGCAGTCCCTCTTGTGTGCTTTTTTAGCGCGGGTGGCCTCCGTG; GGGCAACCCGCCCACCCGTCCGCCCCTCTTTGTCTCCTCCAGTCTTGTACATAATCTCCA; TAGTATGCCCTGAAGTCGTCGCTCCCACCCCCCCGCACCCATTGCGCCCCCTCGCGGTTC; TCCCGCCTCATCTTGCCCCACTCCGTCGTCCCGTCGCGTTTCTCAACCAGTGGCGTTCTC; ACCCCGCATCATTTCACCGCCCCCTCTTGTCCCAGCCCCCTTTGCTCCACCGCGGTTACG; TCAGACCCTCACGTATTCCGCCGTCCGGGCCCTCCCGCTAGCCCACTTGCCGCCCTACG;
• Restriction Sites: NotI-NotI
• ACCN: NM_004360
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004360.2, NP_004351.1
• RefSeq Size: 4815 bp
• RefSeq ORF: 4815 bp
• Locus ID: 999
• UniProt ID: P12830
• Cytogenetics: 16q22.1
• Domains: CA, Cadherin_C_term
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer
• Summary: This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]; Transcript Variant: This variant (1) encodes the longest isoform (1).