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B Raf (BRAF) (NM_004333) Human Over-expression Lysate

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SKU
LY401382
Transient overexpression lysate of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) (as WB positive control)
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211013
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004333, NP_004324
Synonyms B-raf; B-RAF1; BRAF1; NS7; RAFB1
Predicted MW 84.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004324
Locus ID 673
Cytogenetics 7q34
Protein Families WB
Protein Pathways Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction
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Other "B Raf" proteins (13)
SKU Description Size Price
PH311013 BRAF MS Standard C13 and N15-labeled recombinant protein (NP_004324) 10 ug
$3,360.00
LC401382 Lyophilized BRAF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP311013 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), 20 µg 20 ug
$867.00
TP700031 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600E mutant, expressed in human cells 20 ug
$900.00
TP700032 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain, expressed in human cells 20 ug
$900.00
TP700033 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain V600E mutant, expressed in human cells 20 ug
$900.00
TP700044 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600A mutant, expressed in human cells 20 ug
$900.00
TP700046 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600G mutant, expressed in human cells 20 ug
$900.00
TP700047 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600M mutant, expressed in human cells 20 ug
$900.00
TP700048 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600D mutant, expressed in human cells 20 ug
$900.00
TP700049 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600R mutant, expressed in human cells 20 ug
$900.00
TP700050 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600K mutant, expressed in human cells 20 ug
$900.00
TP700051 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) K601E mutant, expressed in human cells 20 ug
$900.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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