B Raf (BRAF) (NM_004333) Human Recombinant Protein
SKU
TP311013
Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 84.3 kDa |
| Concentration | >0.1 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader. |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_004324 |
| Locus ID | 673 |
| UniProt ID | P15056 |
| Cytogenetics | 7q34 |
| RefSeq Size | 2949 |
| RefSeq ORF | 2298 |
| Synonyms | B-raf; B-RAF1; BRAF1; NS7; RAFB1 |
| Summary | This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. provided by RefSeq, Aug 2017 |
| Protein Categories | ADC Targets, Cancer: Endocrine, Cancer: Gastrointestinal, Cancer: Haematopoietic and Lymphoid, Cancer: Lung, Cancer: Skin, Cytokines, Enzyme: Kinases, Growth Factors, Intracellular Proteins |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction |
Reviews
Documents
| FAQs |
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Resources
| Recombinant Protein Resources |
Other "B Raf" proteins (13)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH311013 | BRAF MS Standard C13 and N15-labeled recombinant protein (NP_004324) | 10 ug |
$3,360.00
|
|
| LC401382 | Lyophilized BRAF HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY401382 | Transient overexpression lysate of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) (as WB positive control) | 100 ug |
$436.00
|
|
| TP700031 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600E mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700032 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain, expressed in human cells | 20 ug |
$900.00
|
|
| TP700033 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain V600E mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700044 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600A mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700046 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600G mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700047 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600M mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700048 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600D mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700049 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600R mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700050 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600K mutant, expressed in human cells | 20 ug |
$900.00
|
|
| TP700051 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) K601E mutant, expressed in human cells | 20 ug |
$900.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
