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B Raf (BRAF) (NM_004333) Human Recombinant Protein

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SKU
TP311013M
Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), 100 µg
Size:
100 ug 20 ug 1 mg
  $2,950.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC211013 protein sequence
Red=Cloning site Green=Tags(s)

MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGG
EHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQN
PTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGW
DTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMC
VNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPAD
EDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSP
GPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAP
TPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQT
AQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDK
NPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKK
RDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 84.3 kDa
Concentration >0.1 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader.
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_004324
Locus ID 673
UniProt ID P15056
Cytogenetics 7q34
RefSeq Size 2949
RefSeq ORF 2298
Synonyms B-raf; B-RAF1; BRAF1; NS7; RAFB1
Summary This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. provided by RefSeq, Aug 2017
Protein Categories ADC Targets, Cancer: Endocrine, Cancer: Gastrointestinal, Cancer: Haematopoietic and Lymphoid, Cancer: Lung, Cancer: Skin, Cytokines, Enzyme: Kinases, Growth Factors, Intracellular Proteins
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction
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Documents
Resources

file-alt Citations

ARAF protein kinase activates RAS by antagonizing its binding to RASGAP NF1
Su, Mukherjee, Yaeger et al
Mol Cell (2022) 82 (13), 2443-2457.e7 DOI: 10.1016/j.molcel.2022.04.034
Cited by 23
AI Summary: OriGene products, including Recombinant Human ARAF (TP300737), BRAF (TP311013), CRAF (TP301983), and KRAS (TP301958), were used in this study to investigate how ARAF regulates RAS activation by displacing NF1 and influencing RAS signaling pathways.
Snippet: … We showed here that the Save;Recombinant Human ARAF Origene TP300737 Recombinant Human BRAF Origene TP311013 Recombinant Human CRAF Origene TP301983 Recombinant Human NF1-333 Creative Biomart NF1-334H Recombinant Human EGF Invitrogen PHG0311 Recombinant Rat NGF R & D 7185-NG-025 Recombinant Human KRAS Origene TP301958
New BRAF (v raf murine sarcoma viral oncogene homologue B1) mutation in rheumatoid arthritis
Arnoux, Fina, Lambert et al
Arthritis & rheumatology (2016)
Cited by 4
AI Summary: OriGene's BRAF Val600Ala recombinant protein was used in this study to assess the impact of the newly identified BRAF mutation on kinase activity in vitro.
Snippet: Briefly, MEK1 was incubated for 30 min at 30°C with 0.1 µg wild type BRAF protein, BRAF Val600Ala protein (Origene Technologies, Rockville, USA) and BRAF Val600Glu protein known to be hyperactive (Life technologies, Carlsbad, USA) (12). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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