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B Raf (BRAF) (NM_004333) Human Over-expression Lysate

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SKU
LC401382
Lyophilized BRAF HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211013
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004333, NP_004324
Synonyms B-raf; B-RAF1; BRAF1; NS7; RAFB1
Predicted MW 84.4 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_004324
Locus ID 673
Cytogenetics 7q34
Protein Families WB
Protein Pathways Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction
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Other "B Raf" proteins (13)
SKU Description Size Price
PH311013 BRAF MS Standard C13 and N15-labeled recombinant protein (NP_004324) 10 ug
$3,360.00
LY401382 Transient overexpression lysate of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) (as WB positive control) 100 ug
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TP311013 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), 20 µg 20 ug
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TP700031 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600E mutant, expressed in human cells 20 ug
$900.00
TP700032 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain, expressed in human cells 20 ug
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TP700033 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain V600E mutant, expressed in human cells 20 ug
$900.00
TP700044 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600A mutant, expressed in human cells 20 ug
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TP700046 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600G mutant, expressed in human cells 20 ug
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TP700047 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600M mutant, expressed in human cells 20 ug
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TP700048 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600D mutant, expressed in human cells 20 ug
$900.00
TP700049 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600R mutant, expressed in human cells 20 ug
$900.00
TP700050 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600K mutant, expressed in human cells 20 ug
$900.00
TP700051 Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) K601E mutant, expressed in human cells 20 ug
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file-alt Citations

Acquired BRAF G469A Mutation as a Resistance Mechanism to First-Line Osimertinib Treatment in NSCLC Cell Lines Harboring an EGFR Exon 19 Deletion
La Monica, Minari, Cretella et al
Target Oncol (2019)
AI Summary: OriGene's BRAF G469A (NM_004333) plasmid was used to stably transfect PC9 and HCC827 cells, enabling the authors to demonstrate that the BRAF G469A mutation confers resistance to osimertinib in EGFR-mutant NSCLC cell lines.
Snippet: 2.6 Plasmid Transfection PC9 and HCC827 cells were transfected with 2 µg of BRAF G469A (NM-_004333) [Origene Technologies, Rockville, MD, USA]) using Lipofectamine following the manufac- turer's protocol. Selection of cells was started 24 h later in
Showing 1-1 of 1 papers.
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