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EPCAM (NM_002354) Human Over-expression Lysate

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SKU
LY400847
Transient overexpression lysate of epithelial cell adhesion molecule (EPCAM) (as WB positive control)
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
In Stock*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201989
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002354, NP_002345
Synonyms DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1
Predicted MW 34.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002345
Locus ID 4072
Cytogenetics 2p21
Protein Families WB
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Other "EPCAM" proteins (16)
SKU Description Size Price
PH301989 EPCAM MS Standard C13 and N15-labeled recombinant protein (NP_002345) 10 ug
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LC400847 Lyophilized EPCAM HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP301989 Recombinant protein of human epithelial cell adhesion molecule (EPCAM), 20 µg 20 ug
$737.00
TP710374 Purified recombinant protein of Human epithelial cell adhesion molecule (EPCAM), esidues 24-265aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00
TP720296 Recombinant protein of human epithelial cell adhesion molecule (EPCAM) 10 ug
$220.00
TP721349 Human Trop1/EpCAM Protein (C-His) 25 ug
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TP721350 Human Trop1/EpCAM Protein (C-His-Avi) 25 ug
$300.00
TP721351 Biotinylated Human Trop1/EpCAM Protein (C-His-Avi) 25 ug
$430.00
TP721352 PE Conjugated Human Trop1/EpCAM Protein (C-His) 25 ug
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TP721353 APC Conjugated Human Trop1/EpCAM Protein (C-His) 25 ug
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TP721354 Human Trop1/EpCAM Protein (C-Fc) 25 ug
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TP721355 Human Trop1/EpCAM Protein (C-Fc-Avi) 25 ug
$300.00
TP721356 Biotinylated Human Trop1/EpCAM Protein (C-Fc-Avi) 25 ug
$430.00
TP721357 PE Conjugated Human Trop1/EpCAM Protein (C-Fc) 25 ug
$430.00
TP721358 APC Conjugated Human Trop1/EpCAM Protein (C-Fc) 25 ug
$430.00
TP723976 Recombinant human EPCAM protein with C-terminal 6×His tag 100 ug
$695.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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