CUL4A (NM_001008895) Human Over-expression Lysate
SKU
LY400388
Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 1 (as WB positive control)
$665.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC214798 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001008895, NP_001008895 |
| Predicted MW | 87.7 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001008895 |
| Locus ID | 8451 |
| Cytogenetics | 13q34 |
| Protein Families | WB |
| Protein Pathways | Nucleotide excision repair, Ubiquitin mediated proteolysis |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CUL4A" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303463 | CUL4A MS Standard C13 and N15-labeled recombinant protein (NP_003580) | 10 ug |
$3,360.00
|
|
| LC400388 | Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC418559 | Lyophilized CUL4A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY418559 | Transient overexpression lysate of cullin 4A (CUL4A), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| TP303463 | Purified recombinant protein of Homo sapiens cullin 4A (CUL4A), transcript variant 2, 20 µg | 20 ug |
$737.00
|
|
Citations
Autophagy Plays a Role in the CUL4A-Related Poor Prognosis of Intrahepatic Cholangiocarcinoma
Weng, Liu, Eng et al
Pathol Oncol Res (2021) 27, 602714 DOI: 10.3389/pore.2021.602714
Weng, Liu, Eng et al
Pathol Oncol Res (2021) 27, 602714 DOI: 10.3389/pore.2021.602714
AI Summary: OriGene products, including the CUL4A expression plasmid (RC207392) and CUL4A shRNA (TL312627), were used to manipulate CUL4A levels in intrahepatic cholangiocarcinoma cell lines, enabling the study of CUL4A’s role in regulating autophagy and its association with poor prognosis.
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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