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CD10 (MME) (NM_000902) Human Over-expression Lysate

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SKU
LY400326
Transient overexpression lysate of membrane metallo-endopeptidase (MME), transcript variant 1 (as WB positive control)
  • LY400326 is the same product as LY425018.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $665.00
In Stock*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC223013
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000902, NP_000893
Synonyms CALLA; CD10; CMT2T; NEP; SCA43; SFE
Predicted MW 85.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000893
Locus ID 4311
Cytogenetics 3q25.2
Protein Families WB
Protein Pathways Alzheimer's disease, Hematopoietic cell lineage, Renin-angiotensin system
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Other "CD10" proteins (21)
SKU Description Size Price
PH310961 MME MS Standard C13 and N15-labeled recombinant protein (NP_009219) 10 ug
$3,360.00
PH323013 MME MS Standard C13 and N15-labeled recombinant protein (NP_000893) 10 ug
$3,360.00
PH323061 MME MS Standard C13 and N15-labeled recombinant protein (NP_009218) 10 ug
$3,360.00
PH323116 MME MS Standard C13 and N15-labeled recombinant protein (NP_009220) 10 ug
$3,360.00
LC400326 Lyophilized MME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC416064 Lyophilized MME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC416065 Lyophilized MME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC416066 Lyophilized MME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC425018 Lyophilized MME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429337 Lyophilized MME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429338 Lyophilized MME HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY416064 Transient overexpression lysate of membrane metallo-endopeptidase (MME), transcript variant 1bis (as WB positive control) 100 ug
$665.00
LY416065 Transient overexpression lysate of membrane metallo-endopeptidase (MME), transcript variant 2a (as WB positive control) 100 ug
$436.00
LY416066 Transient overexpression lysate of membrane metallo-endopeptidase (MME), transcript variant 2b (as WB positive control) 100 ug
$665.00
LY425018 Transient overexpression lysate of membrane metallo-endopeptidase (MME), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY429337 Transient overexpression lysate of membrane metallo-endopeptidase (MME), transcript variant 2a (as WB positive control) 100 ug
$665.00
LY429338 Transient overexpression lysate of membrane metallo-endopeptidase (MME), transcript variant 2b (as WB positive control) 100 ug
$665.00
TP310961 Recombinant protein of human membrane metallo-endopeptidase (MME), transcript variant 2a, 20 µg 20 ug
$867.00
TP323013 Recombinant protein of human membrane metallo-endopeptidase (MME), transcript variant 1, 20 µg 20 ug
$867.00
TP323061 Recombinant protein of human membrane metallo-endopeptidase (MME), transcript variant 1bis, 20 µg 20 ug
$867.00
TP323116 Recombinant protein of human membrane metallo-endopeptidase (MME), transcript variant 2b, 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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