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RAB7 (RAB7A) (NM_004637) Human Over-expression Lysate

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SKU
LC417854
Lyophilized RAB7A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201776
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004637, NP_004628
Synonyms CMT2B; PRO2706; RAB7
Predicted MW 23.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_004628
Locus ID 7879
Cytogenetics 3q21.3
Protein Families WB
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Other "RAB7" proteins (3)
SKU Description Size Price
PH301776 RAB7A MS Standard C13 and N15-labeled recombinant protein (NP_004628) 10 ug
$3,360.00
LY417854 Transient overexpression lysate of RAB7A, member RAS oncogene family (RAB7A) (as WB positive control) 100 ug
$436.00
TP301776 Recombinant protein of human RAB7A, member RAS oncogene family (RAB7A), 20 µg 20 ug
$737.00

file-alt Citations

Early to intermediate steps of tumor embolic formation involve specific proteolytic processing of E-cadherin regulated by Rab7
Ye, Gao, Tian et al
Mol. Cancer Res. (2012) 10 (6), 713-26 DOI: 10.1158/1541-7786.MCR-12-0009
AI Summary: OriGene products contributed to this study by providing overexpressed HEK293T lysates for Rab7 (LY417854), calpain 1 (LY401587), and calpain 2 (LY400662), which were used in overexpression experiments to investigate their roles in E-cadherin processing during tumor embolic formation.
Snippet: Overexpression experiments: Rab7, calpain 1, and calpain 2. Rab7-overexpressed, calpain 1- and calpain 2–overexpressed HEK293T lysates (LY417854, LY401587, LY400662, respectively) were purchased (Origene Technologies, Inc.).
Showing 1-1 of 1 papers.
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