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LAPTM4B (NM_018407) Human Over-expression Lysate

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SKU
LC413079
Lyophilized LAPTM4B HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207325
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018407, NP_060877
Synonyms LAPTM4beta; LC27
Predicted MW 35 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_060877
Locus ID 55353
Cytogenetics 8q22.1
Protein Families WB
Protein Pathways Lysosome
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Other "LAPTM4B" proteins (3)
SKU Description Size Price
PH307325 LAPTM4B MS Standard C13 and N15-labeled recombinant protein (NP_060877) 10 ug
$3,360.00
LY413079 Transient overexpression lysate of lysosomal protein transmembrane 4 beta (LAPTM4B) (as WB positive control) 100 ug
$436.00
TP307325 Recombinant protein of human lysosomal protein transmembrane 4 beta (LAPTM4B), 20 µg 20 ug
$867.00

file-alt Citations

Lysosomal-associated transmembrane protein 4B regulates ceramide-induced exosome release
Yuyama, Sun, Mikami et al
FASEB J (2020)
AI Summary: OriGene contributed to this study by providing the C-terminal FLAG-tagged LAPTM4B cDNA clone in the pCMV6 vector, which was used for LAPTM4B re-expression experiments to investigate its role in ceramide-induced exosome release.
Snippet: manufacturer's protocol. All experiments were performed 2 days after transfection. LAPTM4B re‐expression was conducted using a C‐terminal FLAG‐tagged LAPTM4B cDNA clone inserted into pCMV6 vector (Origene). Mutant LAPTM4B
LAPTM4B is associated with poor prognosis in NSCLC and promotes the NRF2-mediated stress response pathway in lung cancer cells
Maki, Fujimoto, Lang et al
Sci Rep (2015) 5, 13846 DOI: 10.1038/srep13846
Cited by 23
AI Summary: OriGene’s C-terminal FLAG-tagged LAPTM4B cDNA clone in the pCMV6 vector was used to overexpress LAPTM4B in lung cancer cells, enabling the authors to study its role in NSCLC progression and NRF2-mediated stress response.
Snippet: to the manufacturer's instructions. LAPTM4B overexpression was achieved using a C-terminal FLAG-tagged LAPTM4B cDNA clone inserted into a cytomegalovirus plasmid pCMV6 vector (Origene). Cells were transfected with ... Cite SaveSaving
FigureFigureFigure
Showing 1-2 of 2 papers.
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