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LAPTM4B (NM_018407) Human Tagged ORF Clone

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SKU
RC207325
LAPTM4B (Myc-DDK-tagged)-Human lysosomal protein transmembrane 4 beta (LAPTM4B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LAPTM4B
Synonyms LAPTM4beta; LC27
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207325 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCACGGACTCGGGTCACATGGCCGAGTCCGCCCCGCCCCCTCCCCGTCCCCGCCGCTGCAGCCG
TCGCCTTCGGAGCGAAGGGTACCGACCCGGCAGAAGCTCGGAGCTCTCGGGGTATCGAGGAGGCAGGCCC
GCGGGCGCACGGGCGAGCGGGCCGGGAGCCGGAGCGGCGGAGGAGCCGGCAGCAGCGGCGCGGCGGGCTC
CAGGCGAGGCGGTCGACGCTCCTGAAAACTTGCGCGCGCGCTAGCGCCACTGCGCCCGGAGCGATGAAGA
TGGTCGCGCCCTGGACGCGGTTCTACTCCAACAGCTGCTGCTTGTGCTGCCATGTCCGCACCGGCACCAT
CCTGCTCGGCGTCTGGTATCTGATCATCAATGCTGTGGTACTGTTGATTTTATTGAGTGCCCTGGCTGAT
CCGGATCAGTATAACTTTTCAAGTTCTGAACTGGGAGGTGACTTTGAGTTCATGGATGATGCCAACATGT
GCATTGCCATTGCGATTTCTCTTCTCATGATCCTGATATGTGCTACGGCTACTTACGGAGCGTACAAGCA
ACGCGCAGCCTGGATCATCCCATTCTTCTGTTACCAGATCTTTGACTTTGCCCTGAACATGTTGGTTGCA
ATCACTGTGCTTATTTATCCAAACTCCATTCAGGAATACATACGGCAACTGCCTCCTAATTTTCCCTACA
GAGATGATGTCATGTCAGTGAATCCTACCTGTTTGGTCCTTATTATTCTTCTGTTTATTAGCATTATCTT
GACTTTTAAGGGTTACTTGATTAGCTGTGTTTGGAACTGCTACCGATACATCAATGGTAGGAACTCCTCT
GATGTCCTGGTTTATGTTACCAGCAATGACACTACGGTGCTGCTACCCCCGTATGATGATGCCACTGTGA
ATGGTGCTGCCAAGGAGCCACCGCCACCTTACGTGTCTGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207325 protein sequence
Red=Cloning site Green=Tags(s)

MTSRTRVTWPSPPRPLPVPAAAAVAFGAKGTDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGL
QARRSTLLKTCARASATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALAD
PDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICATATYGAYKQRAAWIIPFFCYQIFDFALNMLVA
ITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSS
DVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018407
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018407.4, NP_060877.3
RefSeq Size 2242 bp
RefSeq ORF 681 bp
Locus ID 55353
UniProt ID Q86VI4
Cytogenetics 8q22.1
Domains Mtp
Protein Families Transmembrane
Protein Pathways Lysosome
MW 35 kDa
Summary Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).UniProtKB/Swiss-Prot Function
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"NM_018407" in other vectors (6)
SKU Description Size Price
RC207325L1 Lenti ORF clone of Human lysosomal protein transmembrane 4 beta (LAPTM4B), Myc-DDK-tagged 10 ug
$600.00
RC207325L2 Lenti ORF clone of Human lysosomal protein transmembrane 4 beta (LAPTM4B), mGFP tagged 10 ug
$600.00
RC207325L3 Lenti ORF clone of Human lysosomal protein transmembrane 4 beta (LAPTM4B), Myc-DDK-tagged 10 ug
$600.00
RC207325L4 Lenti ORF clone of Human lysosomal protein transmembrane 4 beta (LAPTM4B), mGFP tagged 10 ug
$600.00
RG207325 LAPTM4B (tGFP-tagged) - Human lysosomal protein transmembrane 4 beta (LAPTM4B) 10 ug
$500.00
SC113496 LAPTM4B (untagged)-Human lysosomal protein transmembrane 4 beta (LAPTM4B) 10 ug
$300.00

Plasmid Map for RC207325

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207325
Circular map for RC207325

file-alt Citations

Vpr counteracts the restriction of LAPTM5 to promote HIV-1 infection in macrophages
Zhao, Wang, Xu et al
Nat Commun (2021) 12 (1), 3691 DOI: 10.1038/s41467-021-24087-8
Cited by 44
AI Summary: OriGene contributed LAPTM5, 4A, and 4B expression vectors, which were used in this study to investigate how Vpr counteracts LAPTM5-mediated restriction of HIV-1 infection in macrophages.
Snippet: MG132, chloroquine (CLQ), and bafilomycin A1 (BFLA) were purchased from Sigma Aldrich. Plasmids. The LAPTM5, 4A, and 4B expression vectors were purchased from OriGene, or their open reading frames were de novo cloned into pCMV-3Tag-2A (Addgene) vector
FigureFigureFigure
Lysosomal-associated transmembrane protein 4B regulates ceramide-induced exosome release
Yuyama, Sun, Mikami et al
FASEB J (2020)
AI Summary: OriGene contributed to this study by providing the C-terminal FLAG-tagged LAPTM4B cDNA clone in the pCMV6 vector, which was used for LAPTM4B re-expression experiments to investigate its role in ceramide-induced exosome release.
Snippet: manufacturer's protocol. All experiments were performed 2 days after transfection. LAPTM4B re‐expression was conducted using a C‐terminal FLAG‐tagged LAPTM4B cDNA clone inserted into pCMV6 vector (Origene). Mutant LAPTM4B
LAPTM4B Predicts Axillary Lymph Node Metastasis in Breast Cancer and Promotes Breast Cancer Cell Aggressiveness in Vitro
Xiao, Yang, Meng et al
Cell. Physiol. Biochem. (2017) 41 (3), 1072-1082 DOI: 10.1159/000464115
AI Summary: OriGene’s human LAPTM4B cDNA clone (NM_018407) was used as the template to amplify the complete LAPTM4B open reading frame for functional studies in this paper.
Snippet: The complete open reading frame (ORF) of human LAPTM4B was polymerase chain reaction(PCR)-amplified from a cDNA clone from OriGene (NM_018407) using specific primers to introduce an EcoRI site at the 5′end and an XhoI site at the 3′end and cloned into ... All 2 versions Cite SaveSaving
LAPTM4B is associated with poor prognosis in NSCLC and promotes the NRF2-mediated stress response pathway in lung cancer cells
Maki, Fujimoto, Lang et al
Sci Rep (2015) 5, 13846 DOI: 10.1038/srep13846
Cited by 23
AI Summary: OriGene’s C-terminal FLAG-tagged LAPTM4B cDNA clone in the pCMV6 vector was used to overexpress LAPTM4B in lung cancer cells, enabling the authors to study its role in NSCLC progression and NRF2-mediated stress response.
Snippet: to the manufacturer's instructions. LAPTM4B overexpression was achieved using a C-terminal FLAG-tagged LAPTM4B cDNA clone inserted into a cytomegalovirus plasmid pCMV6 vector (Origene). Cells were transfected with ... Cite SaveSaving
FigureFigureFigure
Showing 1-4 of 4 papers.
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