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LAPTM4B (NM_018407) Human Recombinant Protein

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SKU
TP307325
Recombinant protein of human lysosomal protein transmembrane 4 beta (LAPTM4B), 20 µg
Size:
20 ug 1 mg 100 ug
  $867.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC207325 protein sequence
Red=Cloning site Green=Tags(s)

MTSRTRVTWPSPPRPLPVPAAAAVAFGAKGTDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGL
QARRSTLLKTCARASATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALAD
PDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICATATYGAYKQRAAWIIPFFCYQIFDFALNMLVA
ITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSS
DVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 34.9 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_060877
Locus ID 55353
UniProt ID Q86VI4
Cytogenetics 8q22.1
RefSeq Size 2242
RefSeq ORF 951
Synonyms LAPTM4beta; LC27
Summary Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).UniProtKB/Swiss-Prot Function
Protein Categories Growth Factors, Membrane Proteins
Protein Families Transmembrane
Protein Pathways Lysosome
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Other "LAPTM4B" proteins (3)
SKU Description Size Price
PH307325 LAPTM4B MS Standard C13 and N15-labeled recombinant protein (NP_060877) 10 ug
$3,360.00
LC413079 Lyophilized LAPTM4B HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY413079 Transient overexpression lysate of lysosomal protein transmembrane 4 beta (LAPTM4B) (as WB positive control) 100 ug
$436.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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