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NEDD4 (NM_198400) Human Over-expression Lysate

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SKU
LC405051
Lyophilized NEDD4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212738
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_198400, NP_940682
Synonyms NEDD4-1; RPF1
Predicted MW 140.7 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_940682
Locus ID 4734
Cytogenetics 15q21.3
Protein Families WB
Protein Pathways Endocytosis, Ubiquitin mediated proteolysis
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Other "NEDD4" proteins (5)
SKU Description Size Price
PH321706 NEDD4 MS Standard C13 and N15-labeled recombinant protein (NP_006145) 10 ug
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LC416833 Lyophilized NEDD4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY405051 Transient overexpression lysate of neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY416833 Transient overexpression lysate of neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1 (as WB positive control) 100 ug
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file-alt Citations

Cell surface expression of human ether-a-go-go-related gene (hERG) channels is regulated by caveolin-3 protein via the ubiquitin ligase Nedd4-2
Guo, Wang, Li et al
J. Biol. Chem. (2012) 287 (40), 33132-41 DOI: 10.1074/jbc.M112.389643
Cited by 7
AI Summary: OriGene products contributed to this study by providing the Cav3 plasmid and Myc-tagged Nedd4-1 plasmid, which were used to investigate the molecular interactions regulating hERG channel expression.
Snippet: delays cardiac repolarization and causes long QT syndrome (3). However, an increase in I hERG can cause short QT syndrome (4). Both long QT syndrome and short QT syndrome ... Cav3 plasmid and Myc-tagged plasmid of Nedd4-1 were obtained from Origene Technologies.
Showing 1-1 of 1 papers.
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