NEDD4 (NM_198400) Human Tagged ORF Clone
SKU
RC212738
NEDD4 (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2
$1,085.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NEDD4 |
| Synonyms | NEDD4-1; RPF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_198400 |
| ORF Size | 3741 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_198400.3 |
| RefSeq Size | 7024 bp |
| RefSeq ORF | 3744 bp |
| Locus ID | 4734 |
| UniProt ID | P46934 |
| Cytogenetics | 15q21.3 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| MW | 140.7 kDa |
| Summary | This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. provided by RefSeq, Jul 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_198400" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC212738L1 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,385.00
|
|
| RC212738L2 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged | 10 ug |
$1,385.00
|
|
| RC212738L3 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,385.00
|
|
| RC212738L4 | Lenti ORF clone of Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2, mGFP tagged | 10 ug |
$1,385.00
|
|
| RG212738 | NEDD4 (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 | 10 ug |
$1,285.00
|
|
| SC123396 | NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 2 | 10 ug |
$1,086.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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